AB0100
Alkaline Phosphatase Blue Microwell Substrate
alkaline phosphatase substrate, solution
Synonym(s):
5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Product Name
Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate
form
solution
Quality Level
usage
sufficient for 400 mL working substrate
storage temp.
2-8°C
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General description
Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.
Application
Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.
Physical form
A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Regulatory Information
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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