SHP2Bridge ELISA

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is pre-coated with a panel of 27 antibodies raised against peptides derived from the full length protein sequence of SHP2, a total of 27 columns will be used, each column is pre-coated with one specific antibody covering one region of SHP2 (about 30 amino acids in length). Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of some of them is masked by the three dimensional structure (Higher Order Structure, or HOS) of the protein and only a certain number of the antibodies respond. The result is a histogram which can be likened to a ‘fingerprint′ for correctly folded SHP2. For a testing sample from cancer tissue, cell line or purified SHP2, if the protein is not mutated or the mutations do not impact the SHP2 HOS, the ‘fingerprint′ will match that of wild type SHP2. If the SHP2 mutations or a screened small molecule drug candidate resulted in the alteration of the protein HOS, changes in the ‘fingerprint′ generated by the ELISA will point out differences quantitively, this information may be valuable in the characterization of the SHP2 from cancer patient samples, cancer cell lines, other biological samples or the identification of small molecules that are SHP2 HOS disruptors (allosteric regulators).

For research use only. Not for use in diagnostic procedures.

ELISA kit contains three 96-well microplates and all the components necessary to complete an ELISA test.