B0271
Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse
clone GAL-13, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-β-Galactosidase
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Product Name
Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse, clone GAL-13, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GAL-13, monoclonal
form
buffered aqueous solution
species reactivity
bacteria
technique(s)
dot blot: 1:2,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody produced in mouse may be used with ExtrAvidin-Peroxidase in the dot blot technique on native, purified, or crude β-galactosidase. The antibody was used to detect β-galactosidase by immunofluorescence in paraffin-embedded adult mouse kidney sections. It was used in ELISPOT assays at a dilution of 1:1000.
Biochem/physiol Actions
β-galactosidase enzyme is encoded by lacZ gene of E. coli; it acts on lactose and cleaves it to glucose and galactose. Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody binds with soluble enzyme even when bound to a surface without causing any loss of enzymatic activity. It is used as readout for promoter activity in lacZ transfected cells.
The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-b-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ß-Galactosidase enzyme is encoded by lacZ gene of E. coli.
Immunogen
β-galactosidase from E. coli
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Preparation Note
Prepared by conjugation with ε-aminocaproyl biotin.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
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Jennifer E Snyder-Cappione et al.
Journal of immunology (Baltimore, Md. : 1950), 176(4), 2662-2668 (2006-02-04)
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The 94 C-terminal amino acids of the initiator protein DnaA of Escherichia coli are required and sufficient for specific binding to the cognate DNA binding site. The binding domain contains two potential amphipathic alpha-helices and a third alpha-helix. It represents
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Articles
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
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