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Merck
CN

B0436

Sigma-Aldrich

Anti-BAF57 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Mammalian Chromatin Remodeling Complex BRG1-associated Factor 57, Anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1

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About This Item

MDL number:
MFCD07784600
UNSPSC Code:
12352203

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 57 kDa

species reactivity

human, mouse

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using mouse NIH-3T3 cells fixed with paraformaldehyde-triton
microarray: suitable
western blot: 0.25-0.5 μg/mL using HeLa nuclear cell extracts

UniProt accession no.

Q969G3

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SMARCE1(6605)
mouse ... Smarce1(57376)

Immunogen

synthetic peptide corresponding to amino acids 394-411 of human BAF57. The sequence is conserved in mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

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Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
065K4797

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Jorge Castillo-Robles et al.
Scientific reports, 8(1), 15369-15369 (2018-10-20)
SWI/SNF or BAF chromatin-remodeling complexes are polymorphic assemblies of homologous subunit families that remodel nucleosomes and facilitate tissue-specific gene regulation during development. BAF57/SMARCE1 is a BAF complex subunit encoded in animals by a single gene and is a component of
Oleg Osipovich et al.
Nature immunology, 8(8), 809-816 (2007-06-26)
The assembly of genes encoding antigen receptors is regulated by developmental changes in chromatin that either permit or deny access to a single variable-(diversity)-joining recombinase. These changes are guided by transcriptional promoters and enhancers, which serve as accessibility-control elements in

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