B0939
BAFF active human
Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
Synonym(s):
B lymphocyte stimulator, B-cell-activating factor BAFF, BLyS, Dendritic cell-derived TNF-like molecule, TALL-1, TNF- and APOL-related leukocyte expressed ligand 1
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
BAFF active human, Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
biological source
human
recombinant
expressed in Nicotiana
assay
>97% (SDS-PAGE)
form
lyophilized
potency
≤50 ng/mL ED50
mol wt
18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids)
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... TNFSF13B(10673)
Analysis Note
The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED50 ≤ 50 ng/mL
*activity results may vary with PBMC donors.
ED50 ≤ 50 ng/mL
General description
BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.
Other Notes
Extinction Coefficient: E0.1% = 0.791 (A280 nm)
p.I: 6.03
p.I: 6.03
Physical form
Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.
Preparation Note
Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Rita R Barbosa et al.
Journal of clinical immunology, 34(5), 573-583 (2014-05-09)
B-cell survival and differentiation critically depend on the interaction of BAFF-R and TACI with their ligands, BAFF and APRIL. Mature B-cell defects lead to Common Variable Immunodeficiency (CVID), which is associated with elevated serum levels of BAFF and APRIL. Nevertheless
Xiao-Juan Zhu et al.
British journal of haematology, 166(5), 783-791 (2014-06-04)
To investigate the expression of tumour necrosis factor superfamily 13B (TNFSF13B) receptors in immune thrombocytopenia (ITP) and their correlation with disease activity, we investigated the protein and mRNA levels of TNFSF13B, tumour necrosis factor receptor superfamily 13C (TNFRSF13C), TNFRSF13B and
Nuoyan Zheng et al.
BMC nephrology, 16, 72-72 (2015-05-16)
B cell activating factor belonging to the TNF family (BAFF) is vital for B cell survival, proliferation and activation. Evidence indicates that BAFF is systemically or locally increased in glomerulonephritis (e.g. lupus nephritis, IgA nephropathy). However, the effect of BAFF
J Wild et al.
Leukemia, 29(8), 1676-1683 (2015-02-25)
Natural killer (NK) cells are cytotoxic lymphocytes that substantially contribute to the therapeutic benefit of antitumor antibodies like Rituximab, a crucial component in the treatment of B-cell malignancies. In chronic lymphocytic leukemia (CLL), the ability of NK cells to lyse
Reshmi Parameswaran et al.
Molecular cancer therapeutics, 13(6), 1567-1577 (2014-05-16)
B-cell activating factor receptor (BAFF-R) is expressed on precursor B acute lymphoblastic leukemia (pre-B ALL) cells, but not on their pre-B normal counterparts. Thus, selective killing of ALL cells is possible by targeting this receptor. Here, we have further examined
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