B2170
Bromoperoxidase from Corallina officinalis
lyophilized powder, ≥100 units/mg protein (Lowry)
别名:
BCB, Bromide Peroxidase
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关于此项目
Specific activity:
≥100 units/mg protein (Lowry)
form
lyophilized powder
specific activity
≥100 units/mg protein (Lowry)
composition
Protein, ~10%
solubility
water, ethanol: soluble, violet
foreign activity
Chloroperoxidase <1%
storage temp.
−20°C
Application
Bromoperoxidase from Corallina officinalis may be used for staining brain tissue, nuclei, plant chromosomes, reticulocytes, platelets and reticulated red cells. It may be used for the detection of biochemical molecules and the BCB enzyme assay. The BCB assay is also used industrially in optical data storage.
Biochem/physiol Actions
Bromoperoxidase from Corallina officinalis is a phenoxazine dye. The brilliant cresyl blue (BCB) test determines the activity of glucose-6-phosphate dehydrogenase (G6PDH). The activity of this enzyme is greatest in growing oocytes and declines as oocytes mature. It stains reticulocytes and trichomonads. Bromoperoxidase contains a significant amount of nonheme iron. It is activated by vanadate ions. Maximal activity is achieved with stoichiometric vanadium incorporation .
Physical form
Partially purified, lyophilized powder containing MES buffer salts
Other Notes
One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to monobromochlorodimedon per min at pH 6.4 at 25 °C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)