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Merck
CN

B2170

Bromoperoxidase from Corallina officinalis

lyophilized powder, ≥100 units/mg protein (Lowry)

Synonym(s):

BCB, Bromide Peroxidase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein (Lowry)
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Product Name

Bromoperoxidase from Corallina officinalis, lyophilized powder, ≥100 units/mg protein (Lowry)

form

lyophilized powder

specific activity

≥100 units/mg protein (Lowry)

composition

Protein, ~10%

solubility

water, ethanol: soluble, violet

foreign activity

Chloroperoxidase <1%

storage temp.

−20°C

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Physical form

Partially purified, lyophilized powder containing MES buffer salts

Application

Bromoperoxidase from Corallina officinalis may be used for staining brain tissue, nuclei, plant chromosomes, reticulocytes, platelets and reticulated red cells. It may be used for the detection of biochemical molecules and the BCB enzyme assay. The BCB assay is also used industrially in optical data storage.

Biochem/physiol Actions

Bromoperoxidase from Corallina officinalis is a phenoxazine dye. The brilliant cresyl blue (BCB) test determines the activity of glucose-6-phosphate dehydrogenase (G6PDH). The activity of this enzyme is greatest in growing oocytes and declines as oocytes mature. It stains reticulocytes and trichomonads. Bromoperoxidase contains a significant amount of nonheme iron. It is activated by vanadate ions. Maximal activity is achieved with stoichiometric vanadium incorporation .

Other Notes

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to monobromochlorodimedon per min at pH 6.4 at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物源性产品
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H Yu et al.
Biochemical and biophysical research communications, 160(1), 87-92 (1989-04-14)
A nonheme bromoperoxidase has been purified to homogeneity from the red seaweed Corallina officinalis. Like the corresponding enzyme previously reported from C. pilulifera, this bromoperoxidase contains a significant amount of nonheme iron. However, it is vanadate ion and not iron

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