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Merck
CN

B2170

Sigma-Aldrich

Bromoperoxidase from Corallina officinalis

lyophilized powder, ≥100 units/mg protein (Lowry)

别名:

BCB, Bromide Peroxidase

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About This Item

CAS Number:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

表单

lyophilized powder

质量水平

比活

≥100 units/mg protein (Lowry)

组成

Protein, ~10%

溶解性

water, ethanol: soluble, violet

异质活性

Chloroperoxidase <1%

储存温度

−20°C

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应用

Bromoperoxidase from Corallina officinalis may be used for staining brain tissue, nuclei, plant chromosomes, reticulocytes, platelets and reticulated red cells. It may be used for the detection of biochemical molecules and the BCB enzyme assay. The BCB assay is also used industrially in optical data storage.

生化/生理作用

Bromoperoxidase from Corallina officinalis is a phenoxazine dye. The brilliant cresyl blue (BCB) test determines the activity of glucose-6-phosphate dehydrogenase (G6PDH). The activity of this enzyme is greatest in growing oocytes and declines as oocytes mature. It stains reticulocytes and trichomonads. Bromoperoxidase contains a significant amount of nonheme iron. It is activated by vanadate ions. Maximal activity is achieved with stoichiometric vanadium incorporation [1].

单位定义

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to monobromochlorodimedon per min at pH 6.4 at 25 °C.

外形

Partially purified, lyophilized powder containing MES buffer salts

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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