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Merck
CN

B2661

Anti-fd Bacteriophage−Biotin antibody produced in rabbit

~3.5 mg/mL, IgG fraction of antiserum

Synonym(s):

Anti-fd Bacteriophage

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (i)
Citations:
6
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biological source

rabbit

Quality Segment

conjugate

biotin conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

concentration

~3.5 mg/mL

technique(s)

indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Immunogen

fd bacteriophage

Application

Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays.

Biochem/physiol Actions

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



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