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B3133

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride

Name: <I>N</I><SUB>&#945;</SUB>-Benzoyl-<SC>L</SC>-arginine 4-nitroanilide hydrochloride
Brand: SIGMA

trypsin substrate, chromogenic, ≥99% (TLC), powder, suitable for substrate for trypsin

Synonym(s):

L-BAPA, L-BAPNA, BANI

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About This Item

Empirical Formula (Hill Notation):

C₁₉H₂₂N₆O₄ · HCl

CAS Number:

21653-40-7

Molecular Weight:

434.88

NACRES:

NA.32

PubChem Substance ID:

24891685

UNSPSC Code:

12352204

EC Number:

244-505-6

MDL number:

MFCD00063682

Beilstein/REAXYS Number:

4081878

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Product Name

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride, ≥99% (TLC), suitable for substrate for trypsin

Quality Level

200

assay

≥99% (TLC)

form

powder

solubility

acetone: water (1:1): 50 mg/mL

suitability

suitable for substrate for trypsin

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

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General description

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a substrate for trypsin.

Chromogenic substrate for trypsin and other proteolytic enzymes.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as a substrate:

for chymotrypsin and trypsin activity assay in enzymatic extract (EE) of M. tenellum and pancreatic tissue homogenates
in trypsin and papain standard curve generation
in serine protease and fibrinogenolytic assays of eupolytin1 protein

Other Notes

Similar to B3279, but produced for Sigma.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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In vitro protein digestibility of animal, vegetal and microbial feed ingredients for Macrobrachium tenellum

Nolasco-Soria H, et al.

Latin American Journal of Aquatic Research, 46(3), 157-162 (2018)

Antimicrobial effects of o-cymen-5-ol and zinc, alone & in combination in simple solutions and toothpaste formulations.

Robert L Pizzey et al.

International dental journal, 61 Suppl 3, 33-40 (2011-11-02)

This study aimed to evaluate antimicrobial effects of an o-cymen-5-ol/zinc system. o-Cymen-5-ol and zinc gluconate minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined against Streptococcus mutans, Actinomyces viscosus, Porphyromonas gingivalis, Fusobacterium nucleatum and Candida albicans. Synergy was

Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves

Hamza R, et al.

BMC plant biology, 8(17), 1256-1263 (2018)

Feeding colostrum, its composition and feeding duration variably modify proliferation and morphology of the intestine and digestive enzyme activities of neonatal calves

Blattler U, et al.

The Journal of Nutrition, 131(4), 1256-1263 (2001)

The action of trypsin on synthetic chromogenic arginine substrates

Somorin O, et al.

Journal of Biochemistry, 85(1), 157-162 (1979)

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Global Trade Item Number

SKU	GTIN
B3133-250MG	04061833427408
B3133-100MG	04061833427385
B3133-25MG	04061833427415
B3133-1G	04061833427392

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