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B3279

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride

Name: <I>N</I><SUB>&#945;</SUB>-Benzoyl-<SC>L</SC>-arginine 4-nitroanilide hydrochloride
Brand: SIGMA

proteolytic enzyme substrate, chromogenic, ≥98% (HPLC), powder

Synonym(s):

L-BAPA, L-BAPNA, BANI

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About This Item

Empirical Formula (Hill Notation):

C₁₉H₂₂N₆O₄ · HCl

CAS Number:

21653-40-7

Molecular Weight:

434.88

NACRES:

NA.32

PubChem Substance ID:

24891697

UNSPSC Code:

12352204

EC Number:

244-505-6

MDL number:

MFCD00063682

Beilstein/REAXYS Number:

4081878

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Product Name

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride, ≥98% (HPLC)

Quality Level

200

assay

≥98% (HPLC)

form

powder

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

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General description

Chromogenic substrate for trypsin and other proteolytic enzymes.

Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride (L-BAPNA) is a colorless, chromogenic substrate for proteolytic enzymes such as trypsin, actinidin, and papain.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as substrate:

to measure papain activity in spectrophotometric amidase assay
to measure the inhibitory function of human cystatin C (HCC) on the protease activity of papain by colorimetric assay
for trypsin

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Efficient expression, purification and characterization of native human cystatin C in Escherichia coli periplasm

Zhou Y, et al.

Protein Expression and Purification, 111, 18- 22 (2015)

A chemical approach for detecting sulfenic acid-modified proteins in living cells

Reddie KG, et al.

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He YY, et al.

Peptides, 29(10), 1692- 1699 (2008)

Increased Levels of Antinutritional and/or Defense Proteins Reduced the Protein Quality of a Disease-Resistant Soybean Cultivar.

Daniele O B Sousa et al.

Nutrients, 7(7), 6038-6054 (2015-07-25)

The biochemical and nutritional attributes of two soybean (Glycine max (L.) Merr.) cultivars, one susceptible (Seridó) and the other resistant (Seridó-RCH) to stem canker, were examined to assess whether the resistance to pathogens was related to levels of antinutritional and/or

Proteome of Soybean Seed Exudates Contains Plant Defense-Related Proteins Active against the Root-Knot Nematode Meloidogyne incognita.

Raquel O Rocha et al.

Journal of agricultural and food chemistry, 63(22), 5335-5343 (2015-06-03)

Several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. This study focused on the proteins released in the exudates of soybean

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Global Trade Item Number

SKU	GTIN
B3279-100MG	04061833427569
B3279-1G	04061833427576
B3279-250MG	04061833427583

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