B3795
Brain-derived neurotrophic factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Abrineurin, BDNF
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About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~27 kDa
Impurities:
≤1.00 EU/μg Endotoxin level
Product Name
Brain-derived neurotrophic factor human, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized powder
quality
endotoxin tested
mol wt
~27 kDa
packaging
pkg of 10 μg, pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg Endotoxin level
UniProt accession no.
storage temp.
−20°C
General description
BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
Application
Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.
Biochem/physiol Actions
Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.
Physical form
lyophilized from a sterile 0.2 μm filtered solution with BSA
Analysis Note
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
监管及禁止进口产品
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Lisa Oliver et al.
Stem cells and development, 21(15), 2779-2788 (2012-04-24)
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Differentiation and histological analysis of embryonic stem cell-derived neural transplants in mice.
Y Benninger et al.
Brain pathology (Zurich, Switzerland), 10(3), 330-341 (2000-07-08)
We report here that neural transplantation of in vitro-differentiated embryonic stem (ES) cells provides a versatile strategy for gene transfer into the central nervous system. ES cells were subjected to an optimized in vitro differentiation protocol to obtain embryoid bodies.
W Sun et al.
Neuroscience, 164(4), 1854-1866 (2009-09-26)
Cochlear spiral ganglion neurons (SGN) provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Neurotrophic factor 3 (NT-3) and brain derived neurotrophic factor (BDNF) released from hair cells and supporting cells
Lisa Oliver et al.
PloS one, 6(5), e19820-e19820 (2011-05-19)
Adult mesenchymal stem cells (MSCs) can be maintained over extended periods of time before activation and differentiation. Little is known about the programs that sustain the survival of these cells. Undifferentiated adult human MSCs (hMSCs) did not undergo apoptosis in
Bo Tian et al.
Nature cell biology, 11(2), 211-218 (2009-01-20)
The phosphatidylinositol-3-kinase-like kinase ATM (ataxia-telangiectasia mutated) has a central role in coordinating DNA damage responses, including cell-cycle checkpoint control, DNA repair and apoptosis. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. However, the mechanism
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B3795-5UG | 04061833428436 |
| B3795-10UG | 04061833428412 |
| B3795-1MG | 04061826695388 |
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