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Merck
CN

B3804

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Synonym(s):

5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

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Product Name

BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate

Quality Level

100

form

liquid

storage temp.

2-8°C

Related Categories

General description

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Application

BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.

Other Notes

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCX2289
MKCV4566
MKCT4192
MKCR6740
MKCR2607

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Prafulla Katkar et al.
Metallomics : integrated biometal science, 6(1), 117-125 (2013-11-01)
The ability of oxindolimine copper(II) and zinc(II) complexes, known to have antitumor activity, to inhibit human topoisomerase IB has been tested through enzymatic kinetic assays and molecular docking simulations. These copper and zinc compounds are able to inhibit remarkably the
Yu A Nashchekina et al.
Bulletin of experimental biology and medicine, 163(1), 123-128 (2017-06-06)
Collagen I gels with protein concentrations of 1, 2, and 3.5 mg/ml were prepared and embedded in a porous polylactide scaffold to reduce their contraction. Concentration of the gel did not affect its degradation. Collagen gels promoted the formation of
Stephen P Christiansen et al.
Investigative ophthalmology & visual science, 47(2), 605-613 (2006-01-25)
A common treatment for motility disorders of the extraocular muscles (EOMs) is a resection procedure in which there is surgical shortening of the muscle. This procedure results in rotation of the globe toward the resected muscle, increased resting tension across
Stephen Hearty et al.
Journal of microbiological methods, 66(2), 294-312 (2006-02-07)
A panel of hybridomas was produced using intact Listeria monocytogenes serotype 1/2a cells as the immunogen. An IgG2a monoclonal antibody (mAb) 'mAb2B3' was isolated that reacted with L. monocytogenes but not with a representative panel of related Listeria spp. and
Hermann Ehrlich et al.
Nature chemistry, 2(12), 1084-1088 (2010-11-26)
The minerals involved in the formation of metazoan skeletons principally comprise glassy silica, calcium phosphate or carbonate. Because of their ancient heritage, glass sponges (Hexactinellida) may shed light on fundamental questions such as molecular evolution, the unique chemistry and formation
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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Data Sheet - B3804

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