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B4527

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

Name: 5-Bromo-4-chloro-3-indolyl &#946;-<SC>D</SC>-glucopyranoside
Brand: SIGMA

chromogenic, ≥97%, powder

Synonym(s):

X-Glc, X-glucoside

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₅BrClNO₆

CAS Number:

15548-60-4

Molecular Weight:

408.63

NACRES:

NA.83

PubChem Substance ID:

24891784

UNSPSC Code:

12352204

EC Number:

239-603-0

MDL number:

MFCD00063690

Beilstein/REAXYS Number:

1552603

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Product Name

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, ≥97%

Quality Level

200

assay

≥97%

form

powder

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11-,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-LNNRFACYSA-N

Related Categories

Protein Biology

Proteins & Enzymes

Detection Substrates & Enzymes

General description

A histochemical substrate for β-glucosidase.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品

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Certificates of Analysis (COA)

Lot/Batch Number

0000496167

0000470258

0000424186

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Novel compound for identifying Escherichia coli.

W D Watkins et al.

Applied and environmental microbiology, 54(7), 1874-1875 (1988-07-01)

A new chromogenic compound, 5-bromo-4-chloro-3-indoxyl-beta-D-glucuronide, was found to be useful for the rapid, specific, differential identification of Escherichia coli in the sanitary analysis of shellfish and wastewater. Of 1,025 presumptively positive colonies (blue) and 583 presumptively negative colonies (nonblue), only

Novel selective and non-selective optical detection of microorganisms.

L A Shelef et al.

Letters in applied microbiology, 25(3), 202-206 (1997-11-14)

A new instrument, capable of detecting metabolic changes due to microbiological activity, is described. Optical changes in growth media are monitored in a semi-fluid zone that separates the liquid medium containing the sample. Data demonstrate that common media can be

Identification of Bradyrhizobium elkanii USDA61 Type III Effectors Determining Symbiosis with Vigna mungo.

Hien P Nguyen et al.

Genes, 11(5) (2020-05-01)

Bradyrhizobium elkanii USDA61 possesses a functional type III secretion system (T3SS) that controls host-specific symbioses with legumes. Here, we demonstrated that B. elkanii T3SS is essential for the nodulation of several southern Asiatic Vigna mungo cultivars. Strikingly, inactivation of either

Histochemical detection of alpha-D-glucosidases and their molecular forms with 5-Br-4-Cl-3-indoxyl-alpha-D-glucoside.

R Gossrau et al.

Histochemistry, 86(4), 397-404 (1987-01-01)

5-Br-4-Cl-3-Indoxyl-alpha-D-gluco(pyrano)side was found to be the most suitable synthetic substrate for the demonstration of alpha-D-glucosidases in situ. Using an azoindoxyl procedure with hexazotized pararosaniline or new fuchsine at pH 5 in freeze-dried celloidine-mounted cryostat sections acid alpha-D-glucosidase (EC 3.2.1.20) was

Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay.

H Arakawa et al.

Analytical biochemistry, 199(2), 238-242 (1991-12-01)

Chemiluminescent assays of various enzymes have been developed using indoxyl derivatives as substrates. The principle of the method is as follows: an enzyme causes hydrolysis of an indoxyl derivative to an intermediate indoxyl that is readily oxidized to indigo dye

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Global Trade Item Number

SKU	GTIN
B4527-5MG	04061833429730
B4527-100MG	04061833429723
B4527-25MG	04061833267806

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