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Merck
CN

B4875

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

trypsin substrate, chromogenic, elastase substrate, ≥98%, powder

Synonym(s):

Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride, BANI, BAPNA

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About This Item

Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
911-77-3
Molecular Weight:
434.88
NACRES:
NA.32
PubChem Substance ID:
24891801
UNSPSC Code:
12352204
EC Number:
213-011-2
MDL number:
MFCD00012846
Beilstein/REAXYS Number:
4081878

Key Documents

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Product Name

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%

InChI key

DEOKFPFLXFNAON-UHFFFAOYSA-N

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H

SMILES string

Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

assay

≥98%

form

powder

color

, white to light yellow to light brown

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

storage temp.

−20°C

Quality Level

200

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Application

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride has been used as a substrate for trypsin and elastase in enzyme analysis.

General description

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.
A chromogenic trypsin substrate.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4338
BCCN1610
BCCN0290
BCCL6610
BCCK8515

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