B5283
Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal
clone RG-96, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Rabbit IgG (γ-chain specific)
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
RG-96, monoclonal
Application:
ELISA (d), IHC (p), WB CL
Citations:
28
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RG-96, monoclonal
form
buffered aqueous solution
species reactivity
rabbit
should not react with
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
technique(s)
direct ELISA: 1:150,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, western blot (chemiluminescent): 1:200,000-1:400,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
General description
IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother′s milk. The variable region of IgG antibody is specific to antigens and is highly conserved.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Application
Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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María Asunción Sánchez-Gil et al.
International journal of molecular sciences, 25(10) (2024-05-25)
Chronic venous disease (CVD) comprises a spectrum of morphofunctional disorders affecting the venous system, affecting approximately 1 in 3 women during gestation. Emerging evidence highlights diverse maternofetal implications stemming from CVD, particularly impacting the placenta. While systemic inflammation has been
Miguel A Ortega et al.
Biomolecules, 13(1) (2023-01-22)
Psychosis is a complex entity characterized by psychological, behavioral, and motor alterations resulting in a loss of contact with reality. Although it is not common, pregnancy can be a period in which a first episode of psychosis can manifest, entailing
Miguel A Ortega et al.
Cells, tissues, organs, 206(4-5), 272-282 (2019-06-17)
Chronic venous disorder (CVeD), is a disorder in which there is a modification in the conditions of blood return to the heart. The disorder may arise from incompetent valves and the resultant venous reflux (chronic venous insufficiency, CVI). The economic
β-Catenin Signaling Promotes Proliferation of Progenitor Cells in the Adult Mouse Subventricular Zone.
Kazuhide A, et al.
Stem Cells, 25(11), 2827-2836 (2007)
Fabiana Evaristo-Mendonça et al.
Stem cells international, 2019, 7692973-7692973 (2019-09-19)
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are dynamic cells that can sense the environment, adapting their regulatory functions to different conditions. Accordingly, the therapeutic potential of BM-MSCs can be modulated by preconditioning strategies aimed at modifying their paracrine action. Although
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B5283-.2ML | 04061838352552 |
| B5283-.5ML | 04061837514838 |
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