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B5388

Brilliant Cresyl Blue ALD

Dye content, ≥60%, certified by the Biological Stain Commission, powder

Synonym(s):

3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C, Brilliant Cresyl blue

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About This Item

Linear Formula:
C17H20N3OCl · 1/2ZnCl2
CAS Number:
51716-96-2
Molecular Weight:
385.96
NACRES:
NA.47
PubChem Substance ID:
24891836
UNSPSC Code:
12171500
EC Number:
279-675-0
MDL number:
MFCD00148901
Beilstein/REAXYS Number:
5690713
Colour Index Number:
51010
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Product Name

Brilliant Cresyl Blue ALD, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥60%

solubility

water: 1 mg/mL, blue to very deep blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Zn-2](Cl)(Cl)(Cl)Cl.N(CC)(CC)c4cc5[o+]c6c(nc5cc4)cc(c(c6)N)C.N(CC)(CC)c1cc2[o+]c3c(nc2cc1)cc(c(c3)N)C

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

SIHASHFMDFXGSX-UHFFFAOYSA-J

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Application

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).
Certified as a supravital stain for reticulocytes.

Biochem/physiol Actions

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS0898
SHBR5133
SHBQ4266
SHBM9411
127K3729

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Vitrification of immature and matured bovine oocytes: effect of brilliant cresyl blue selection and hyaluronan addition.
Animal Reproduction, 13 (2016)
Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation.
Rodriguez-Gonzalez E, et al.
Reproduction, Nutrition, Development, 43, 179-179 (2003)
Cheesbrough M
District Laboratory Practice in Tropical Countries (2006)
Liqin Wang et al.
Journal of biomedicine & biotechnology, 2012, 161372-161372 (2012-06-08)
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can
A Zh Baltabekova et al.
Molecular biotechnology, 57(8), 746-755 (2015-04-04)
Immunoglobulin E (IgE) plays a central role in type I hypersensitivity including allergy and asthma. Novel treatment strategy envisages development of a therapeutic vaccine designed to elicit autologous blocking antibodies against the IgE. We sought to develop an IgE-epitope antigen
View All Related Papers

Global Trade Item Number

SKUGTIN
B5388-10G04061833433805

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