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Merck
CN

B7059

Monoclonal Anti-Breast Cancer Resistance Protein antibody produced in mouse

~250 μg/mL, clone BXP-21, tissue culture supernatant, aqueous solution

Synonym(s):

Anti-ABCG2, Anti-BCRP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03791618
Conjugate:
unconjugated
Clone:
BXP-21, monoclonal
Application:
FACS, ICC, IHC (f), IHC (p), WB
Citations:
2

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

BXP-21, monoclonal

form

aqueous solution

mol wt

antigen ~70 kDa

species reactivity

human

concentration

~250 μg/mL

technique(s)

flow cytometry: suitable, immunocytochemistry: 1:20-1:50 using acetone-fixed cytospin preparations, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using pretreated human tissue, immunohistochemistry (frozen sections): 1:20 using acetone-fixed frozen sections, western blot: suitable

isotype

IgG2a

UniProt accession no.

Q9UNQ0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ABCG2(9429)

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Immunogen

fusion protein containing human BCRP (amino acids 271-396) and maltose-binding protein.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Reacts with an internal epitope of BCRP. Does not cross-react with the human MDR1, MRP1, MRP2 gene products.

Physical form

Supplied in serum-free medium containing 0.7% bovine serum albumin and 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCD9271
SLBX1798
SLBL6466V
SLBK4403V
SLBF7613

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Cathrine Elisabeth Olsen et al.
Biochemical pharmacology, 144, 63-77 (2017-08-09)
Here we report on the induction of resistance to photodynamic therapy (PDT) in the ABCG2-high human breast cancer cell line MA11 after repetitive PDT, using either Pheophorbide A (PhA) or di-sulphonated meso-tetraphenylchlorin (TPCS2a) as photosensitizer. Resistance to PhA-PDT was associated
Hong Qi et al.
Experimental eye research, 86(1), 34-40 (2007-11-06)
Nerve growth factor (NGF), a member of the neurotrophin family, has been identified as an essential growth factor supporting stem cell self-renewal outside the nervous system and was previously shown to stimulate corneal epithelial proliferation both in vivo and in

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培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。

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