B8400
Monoclonal Anti-Bovine IgG2 antibody produced in mouse
clone BG2-7, ascites fluid
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About This Item
Conjugate:
unconjugated
Clone:
BG2-7, monoclonal
Application:
DB, ELISA (c), ELISA (i), WB
Citations:
4
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
BG2-7, monoclonal
contains
15 mM sodium azide
technique(s)
capture ELISA: suitable, dot blot: suitable, indirect ELISA: 1:1,000, western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
The IgG class antibodies contain two subclasses, IgG1 and IgG2, which have antigenic differences in the Fc portion of the heavy chains. Monoclonal Anti-Bovine IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse.
Immunogen
Purified bovine IgG
Application
Monoclonal Anti-Bovine IgG2 antibody has been used in:
- western blot
- enzyme linked immunosorbent assay (ELISA)
- dot blot assay
Biochem/physiol Actions
Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds
Fernandez B, et al.
Veterinary Medicine International, 2012 (2012)
Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration
Hurley IP, et al.
Journal of Dairy Science, 87(3), 543-549 (2004)
Macarena Sarli et al.
PloS one, 15(2), e0229301-e0229301 (2020-02-23)
Anaplasma marginale is the most prevalent tick-borne livestock pathogen with worldwide distribution. Bovine anaplasmosis is a significant threat to cattle industry. Anaplasmosis outbreaks in endemic areas are prevented via vaccination with live A. centrale produced in splenectomized calves. Since A.
Tom N McNeilly et al.
Vaccine, 28(5), 1412-1421 (2009-11-21)
Systemic immunization of cattle with H7 flagellin results in induction of both H7-specific IgA and IgG antibodies but only partially protects against subsequent colonization with Escherichia coli O157:H7. Recent studies indicate that anti-flagellin antibodies directed against TLR5 binding domains located
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