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B8931

Magenta-Gal

Name: Magenta-Gal
Brand: SIGMA

reagent for selection of recombinant bacterial clones

Synonym(s):

Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₅BrClNO₆

CAS Number:

93863-88-8

Molecular Weight:

408.63

NACRES:

NA.85

PubChem Substance ID:

329773145

UNSPSC Code:

12352200

MDL number:

MFCD02683897

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grade

Molecular Biology

sterility

non-sterile

product line

BioReagent

assay

≥98.0% (HPLC)

form

powder

solubility

methanol: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1

InChI key

CHRVKCMQIZYLNM-HTOAHKCRSA-N

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General description

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.

Biochem/physiol Actions

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos

In Situ Hybridization Methods, 151-167 (2015)

Forward signaling by EphB1/EphB2 interacting with ephrin-B ligands at the optic chiasm is required to form the ipsilateral projection.

George Chenaux et al.

The European journal of neuroscience, 34(10), 1620-1633 (2011-11-23)

EphB receptor tyrosine kinases direct axonal pathfinding through interactions with ephrin-B proteins following axon-cell contact. As EphB:ephrin-B binding leads to bidirectional signals, the contributions of signaling into the Eph-expressing cell (forward signaling) or the ephrin-expressing cell (reverse signaling) cannot be

Characterization of a heat resistant beta-glucosidase as a new reporter in cells and mice.

Susan C McCutcheon et al.

BMC biology, 8, 89-89 (2010-06-24)

Reporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum

Zic1 controls placode progenitor formation non-cell autonomously by regulating retinoic acid production and transport.

Maria Belen Jaurena et al.

Nature communications, 6, 7476-7476 (2015-06-24)

All cranial placode progenitors arise from a common precursor field anterior to the neural plate, the pre-placodal region (PPR). We showed that transcription factor Zic1, expressed at the anterior neural plate, is necessary and sufficient to promote placode fate. Here

Xenopus laevis FGF receptor substrate 3 (XFrs3) is important for eye development and mediates Pax6 expression in lens placode through its Shp2-binding sites.

Yeon-Jin Kim et al.

Developmental biology, 397(1), 129-139 (2014-12-03)

Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and

Global Trade Item Number

SKU	GTIN
B8931-25MG	04061833074022

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