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Merck
CN

BX-0700-30

Human Striatal Medium Spiny GABAergic Neurons

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Human iPSC line, Fully differentiated, Healthy Male Control (no known neurological disorders), Cryopreserved

Synonym(s):

Medium Spiny Neurons (MSNs)

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biological source

human (iPSC line)

form

frozen liquid

packaging

vial of 1 (contains ≥1 million cells)

technique(s)

cell culture | mammalian: suitable

storage temp.

-140 to -196°C

General description

Human iPSC-Derived Striatal Medium Spiny GABAergic Neurons. Required products (sold separately): BX-2400-100uL, BX-2020-100uL, BX-2040-100uL. Spiny striatal GABAergic neurons are inhibitory neurons of a striatal lineage that are found in the striatum. These neurons release GABA as their primary inhibitory neurotransmitter and are important for proper neurologic function. Related diseases: Alzheimer’s Disease, Huntington’s Disease & Schizophrenia.

Application

Calcium Influx Assays: Changes in calcium concentration are closely tied to neuronal activity as action potentials are associated with large pre-synaptic calcium influx and a notable rise in postsynaptic calcium at excitatory synapses. This can be observed experimentally by stimulating the neurons or culturing the neurons under suitable conditions to form mature networks that exhibit spontaneous oscillations. The influx of calcium can be measured using a variety of calcium-sensitive fluorescent dyes, which are commercially available. MEA Assays: Multi-electrode arrays (MEA) measure extracellular voltage changes that occur as neurons fire action potentials. These measurements reveal the firing patterns of individual neurons as well as the patterns of neuronal networks that exist in the cell culture. Such measurements are non-invasive and allow for repeated recordings.

Features and Benefits

Marker expression: Fully differentiated spiny striatal GABAergic neurons that express GABA/DARPP32/MAP2 at DIV 7 with spontaneous firing by DIV 21.

Regulatory Information

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Naoki Iwamoto et al.
Molecular therapy. Nucleic acids, 35(3), 102246-102246 (2024-07-19)
Huntington's disease (HD) is an autosomal dominant disease caused by the expansion of cytosine-adenine-guanine (CAG) repeats in one copy of the HTT gene (mutant HTT, mHTT). The unaffected HTT gene encodes wild-type HTT (wtHTT) protein, which supports processes important for
Kenshiro Fujise et al.
NPJ Parkinson's disease, 11(1), 16-16 (2025-01-10)
The dysfunction of dopaminergic (DA) neurons is central to Parkinson's disease. Distinct synaptic vesicle (SV) populations, differing in neurotransmitter content (dopamine vs. glutamate), may vary due to differences in trafficking and exocytosis. However, the structural organization of these vesicles remains

Protocols

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