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C0662

6-Carboxyfluorescein

~97% purity (HPLC), powder

Synonym(s):

6-FAM, FLUOS

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About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
3301-79-9
Molecular Weight:
376.32
NACRES:
NA.47
PubChem Substance ID:
24892266
UNSPSC Code:
12171500
MDL number:
MFCD00036873
Beilstein/REAXYS Number:
54341
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Product Name

6-Carboxyfluorescein, ≥96% (HPLC)

Quality Level

200

assay

≥96% (HPLC)

form

powder

technique(s)

titration: suitable

color

dark yellow to dark orange

mp

>300 °C (lit.)

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange

λmax

492 nm at 0.1 N in NaOH

fluorescence

λex 492 nm; λem 517 nm (pH 8.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1

InChI

1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

BZTDTCNHAFUJOG-UHFFFAOYSA-N

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Related Categories

Application

6-Carboxyfluorescein has been used to check the plasma membrane integrity in sperm cells.
Fluorescent dye.

Biochem/physiol Actions

6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000504009
0000504009
0000476098
0000476098
0000338459

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Przemysław Płociński et al.
Nature communications, 8(1), 1251-1251 (2017-11-02)
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Villaverde AI, et al.
Animal Reproduction Science, 114, 434-434 (2009)
Evgeny A Semchenko et al.
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Food and environmental virology, 11(3), 297-308 (2019-04-21)
Mismatches between template sequences and reverse transcription (RT) or polymerase chain reaction (PCR) primers can lead to underestimation or false negative results during detection and quantification of sequence-diverse viruses. We performed an in silico inclusivity analysis of a widely used
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Global Trade Item Number

SKUGTIN
C0662-50MG04061832346441

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