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Merck
CN

C0712

Sigma-Aldrich

CDP-Star® Chemiluminescent Substrate

0.25 mM in H2O

Synonym(s):

Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate

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About This Item

Empirical Formula (Hill Notation):
C18H19Cl2O7Na2P
CAS Number:
160081-62-9
Molecular Weight:
495.20
MDL number:
MFCD02094611
UNSPSC Code:
12352204
PubChem Substance ID:
329774818
NACRES:
NA.83

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Assay

≥75% (HPLC)

Quality Level

200

form

liquid

usage

sufficient for 20 blots detection of alkaline phosphatase-labeled molecules (10 × 10 cm)

concentration

0.25 mM in H2O

storage temp.

2-8°C

SMILES string

Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3

InChI

1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;

InChI key

UKWLRLAKGMZXJC-VCCHNUGISA-L

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Application

CDP-Star® is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules in Northern, Southern, and Western blotting applications. Detection of alkaline phosphatase-labeled molecules with CDP-Star is extremely sensitive as a result of low background luminescence coupled with high intensity and prolonged light output from the enzyme catalysis. Following exposure to alkaline phosphatase-labeled molecules, maximum light emission occurs at approximately 60 minutes and continues for up to 24 hours, allowing for multiple film exposures and/or the sensitive detection of targets present in small amounts. For convenience, CDP-Star is supplied as a 0.25 mM, ready-to-use aqueous solution (i.e. no dilution is necessary). CDP-Star functions on both neutral and positively-charged nylon, giving the reagent added application versatility.

Features and Benefits

CDP-Star® is supplied as a 0.25 mM ready-to-use aqueous solution for use in a variety of membrane-based applications. CDP-Star functions on both neutrally-charged and positively-charged nylon, giving the reagent added application versatility.

Legal Information

CDP-Star® is a trademark of Tropix, Inc. Bedford, MA, USA and covered under U.S. Patent 5,326,882.
CDP-Star is a registered trademark of Tropix, Inc.

Disclaimer

Store at 2-8 °C protected from light.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000498763
0000498763
0000490261
0000490261
0000389856

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