C0875
Calcein
Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
description
mixed isomers
form
powder
technique(s)
titration: suitable
color
yellow to brown
solubility
NaOH: 50 mg/mL
fluorescence
λex 470 nm; λem 509 nm (pH 7.4)
suitability
suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O
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Related Categories
Application
- in whole cell staining
- in histomorphometry and micro-computed tomography
- to label active bone-forming surfaces
Biochem/physiol Actions
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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705578-5MG-PW
PL860-CGA/SHF-1EA
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1000309185
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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
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For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What are the excitation and emission wavelengths of Calcein, Product C0875?
Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.
Is there a reference for using Calcein, Product C0875, to study bone growth?
Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).
What is the extinction coefficient of Calcein, Product C0875?
For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.
What is the solubility of Calcein, Product C0875?
Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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