C0979
Anti-Catalase antibody ,Mouse monoclonal
clone CAT-505, purified from hybridoma cell culture
Synonym(s):
Anti-CAT
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About This Item
Conjugate:
unconjugated
Clone:
CAT-505, monoclonal
Application:
ARR, ELISA (i), ICC, IHC, WB
Citations:
83
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CAT-505, monoclonal
form
buffered aqueous solution
mol wt
antigen 60 kDa
species reactivity
human, mouse, bovine, rat
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CAT(847)
mouse ... Cat(12359)
rat ... Cat(24248)
General description
Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa.
Immunogen
human erythrocyte catalase
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (5 papers)
Western Blotting (5 papers)
Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following:
- Immunocytochemistry
- Immunohistochemistry
- Indirect ELISA
- Microarray
- Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells
- Immunofluorescence
Biochem/physiol Actions
Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses.
Physical form
Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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J M Matés et al.
Frontiers in bioscience : a journal and virtual library, 4, D339-D345 (1999-03-17)
Aerobic organisms possess antioxidant defense systems that deal with reactive oxygen species (ROS) produced as a consequence of aerobic respiration. Reactive oxygen is related to both, the arrest of growth and the start of cell differentiation. Low concentrations of reactive
E C Kaal et al.
Journal of neuroscience research, 54(6), 778-786 (1998-12-18)
Evidence is growing that reactive oxygen species (ROS), by-products of (normal) cellular aerobic metabolism, are involved in the pathogenesis of neurodegenerative diseases. One of these diseases is amyotrophic lateral sclerosis (ALS), in which motoneurons die, leading to paralysis and death.
S Lapointe et al.
Biology of reproduction, 58(3), 747-753 (1998-03-25)
Hydrogen peroxide (H2O2) is a reactive oxygen species that at low concentration is toxic to sperm. H2O2 inhibits not only sperm viability but also the acrosome reaction, sperm-egg binding, and oocyte penetration. Catalase activates the decomposition of H2O2 into water
Kisuk Min et al.
Skeletal muscle, 7(1), 21-21 (2017-10-20)
The mitogen-activated protein kinases (MAPKs) have been shown to be involved in regulating myofiber survival. In skeletal muscle, p38 MAPK and JNK are negatively regulated by MAPK phosphatase-5 (MKP-5). During muscle regeneration, MKP-5 is downregulated, thereby promoting p38 MAPK/JNK signaling
K Aoshiba et al.
Journal of immunology (Baltimore, Md. : 1950), 162(3), 1692-1700 (1999-02-11)
Neutrophils constitutively undergo apoptosis at both normal and inflamed sites: an important process that limits the toxic potential of the neutrophil. However, the signal pathway for neutrophil apoptosis is currently unknown. In this study, we evaluated the role of p38-mitogen-activated
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0979-.2ML | 04061833452820 |
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