C0992
Anti-Cy3/Cy5 antibody, Mouse monoclonal
clone CY-96, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Cy3/Cy5, Cy3 Antibody, Cy3 Antibody - Anti-Cy3/Cy5 antibody, Mouse monoclonal
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
CY-96, monoclonal
Application:
direct ELISA
dot blot
immunocytochemistry
immunoprecipitation (IP)
microarray
dot blot
immunocytochemistry
immunoprecipitation (IP)
microarray
Technique(s):
direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
Citations:
10
Product Name
Anti-Cy3/Cy5 antibody, Mouse monoclonal, clone CY-96, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CY-96, monoclonal
form
buffered aqueous solution
concentration
~1.5 mg/mL
technique(s)
direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in:
- immunofluorescence
- western blot
- dot blot
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
- protein microarrays
- In in situ hybridization
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Biochem/physiol Actions
Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
The antibody recognizes Cy3 and Cy5 conjugated to proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5.
Immunogen
mixture of proteins labeled with Cy3/Cy5.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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A K Kenworthy
Methods (San Diego, Calif.), 24(3), 289-296 (2001-06-14)
Fluorescence resonance energy transfer (FRET) detects the proximity of fluorescently labeled molecules over distances >100 A. When performed in a fluorescence microscope, FRET can be used to map protein-protein interactions in vivo. We here describe a FRET microscopy method that
An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nagai-Okatani C, et al.
International Journal of Molecular Sciences, 20(3), 700-700 (2019)
G Enders
Acta neurochirurgica. Supplement, 89, 9-13 (2004-09-01)
Microarray analysis has been emerged as a tool to characterize the overall reaction of cells in culture or tissue to different stimuli e.g. stressful events by analysing bulk RNA present at a particular time point. It has supplemented or even
Transplantation of ovarian granulosa-like cells derived from human induced pluripotent stem cells for the treatment of murine premature ovarian failure
Liu T. et al.
Molecular Medicine Reports, 13(6), 5053-5058 (2016)
Pier Giorgio Righetti et al.
European journal of mass spectrometry (Chichester, England), 10(3), 335-348 (2004-06-10)
The present review attempts to cover the vast array of methods which have appeared in the last few years for performing quantitative proteome analysis. These methods are divided into two classes: those applicable to conventional two-dimensional map analysis, coupling orthogonally
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