C-12640
Human Bronchial Epithelial Cells (HBEpC)
500,000 cryopreserved cells
Synonym(s):
HBEpC cells
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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Product Name
Human Bronchial Epithelial Cells (HBEpC), 500,000 cryopreserved cells
biological source
human bronchi
packaging
pkg of 500,000 cells
morphology
(epithelial)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Analysis Note
Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Application
Primary Human Bronchial Epithelial Cells (HBEpC) are isolated from the surface epithelium of human bronchi and are provided in a cryopreserved format. HBEpC stain positive for cytokeratin. The respiratory epithelia are responsible for the lubrication of the lungs, the maintenance of humidity, and the cleaning of the respiratory tract. They are an important target for drugs, toxins, and carcinogens. In addition, they are involved in many diseases such as cystic fibrosis. Consequently, HBEpC are useful for investigating the function and pathology of the respiratory system.
Biochem/physiol Actions
Bronchi
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our HBEpC are now also available from Air-Liquid Interface pre-screened, HLA-typed, and COPD/Asthma donors.
Our HBEpC are now also available from Air-Liquid Interface pre-screened, HLA-typed, and COPD/Asthma donors.
Other Notes
Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link
Preparation Note
Click here for more information.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Annika Hunziker et al.
Cell reports, 38(4), 110306-110306 (2022-01-27)
Binding of influenza virus to its receptor triggers signaling cascades that reprogram the cell for infection. To elucidate global virus-induced changes to the cellular signaling landscape, we conducted a quantitative phosphoproteomic screen with human and avian influenza viruses. Proteins with
Articles
Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.
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