Key Documents

View All Documentation

C-12720

Human Osteoblasts (HOB)

Name: Human Osteoblasts (HOB)
Brand: SIGMA

500,000 cryopreserved cells

Synonym(s):

HOB cells

Select a Size

Change View

About This Item

NACRES:

NA.81

UNSPSC Code:

41106514

Biological source:

human bone (knee and hip joints)

Morphology:

(osteoblasts)

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

human bone (knee and hip joints)

packaging

pkg of 500,000 cells

morphology

(osteoblasts)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Description

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Application

Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request) and are provided in a cryopreserved format. They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.Osteoblasts are of mesenchymal origin and are mainly responsible for bone formation and remodeling. In vivo, they produce a collagen type I rich extracellular matrix called the osteoid. During their development, osteoblasts undergo a maturation process called osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.

Biochem/physiol Actions

Bone Tissue

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Human Osteoblasts. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, histochemical tests for alkaline phosphatase and bone mineralization are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions lacking antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: Alkaline phosphatase positive, Mineralization testedGuaranteed population doublings: >10

Recommended Primary Cell Culture Media:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Still not finding the right product?

Explore all of our products under Human Osteoblasts (HOB)

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Introduction to Primary Cell Culture

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Primary Cell Culture Basics

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

View All Articles