C-14012
Human Umbilical Vein Endothelial Cells (HUVEC)
Pre-screened, 1 million cells per pellet
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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human umbilical cord vein
Morphology:
(endothelial)
biological source
human umbilical cord vein
packaging
pkg of 1,000,000 cells
morphology
(endothelial)
application(s)
cell analysis (DNA, RNA, and protein)
shipped in
dry ice
storage temp.
−20°C
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.
Application
Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from the vein of the umbilical cord. They are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. HUVEC are isolated from single donors in standard Endothelial Cell Growth Medium. The cells have been pre-screened for VEGF (vascular endothelial growth factor) response. VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis, cell migration, sprouting, and microvascular permeability.
Biochem/physiol Actions
Vein
Physical form
Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.
Preparation Note
Click here for more information.
Analysis Note
Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.
Legal Information
RNAlater is a registered trademark of Ambion, Inc.
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
Primary cells from PromoCell are available in pelleted form for research applications like gene expression analysis, and cell/protein characterization experiments.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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