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Merck
CN

C1427

Cytochrome b5 human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

Synonym(s):

Cytochrome b5

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About This Item

UNSPSC Code:
12161501
NACRES:
NA.47
MDL number:
MFCD02687283
Form:
solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (histidine-tagged)
Mol wt:
16 kDa

Key Documents

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Product Name

Cytochrome b5 human, recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

biological source

human

recombinant

expressed in E. coli (histidine-tagged)

assay

≥90% (SDS-PAGE)

form

solution

mol wt

16 kDa

technique(s)

inhibition assay: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00167

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

human ... CYB5A(1528)

Analysis Note

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b5.

Biochem/physiol Actions

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.
Cytochrome b5 is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.
Membrane-bound heme-containing protein; enhances catalytic efficiency of some P450 isoforms.

General description

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

Physical form

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
SLCN2292
SLCN9704
SLCL7974
SLCH6463
SLCD6016

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Melissa R Pitman et al.
Scientific reports, 12(1), 454-454 (2022-01-12)
Sphingosine 1-phosphate (S1P) is a signaling lipid that has broad roles, working either intracellularly through various protein targets, or extracellularly via a family of five G-protein coupled receptors. Agents that selectively and specifically target each of the S1P receptors have
CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women
Stark K, et al.
Arthritis Research & Therapy, 17(1), 56-56 (2015)
Human adrenal cells that express both beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production
Nakamura Y, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 123(3-5), 122-126 (2011)

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