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C1794

Sigma-Aldrich

Cellulase from Trichoderma sp.

greener alternative

BioReagent, suitable for plant cell culture, 3-10 units/mg solid

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Synonym(s):
β-1,4-glucanase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.72

biological source

fungus (Trichoderma sp.)

Quality Level

product line

BioReagent

form

powder

specific activity

3-10 units/mg solid

contains

maltodextrin

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

cell culture | plant: suitable

application(s)

agriculture

greener alternative category

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Cellulase (β-1,4-glucanase) is a natural enzyme produced by the filamentous fungi Trichoderma viride which promotes the endohydrolysis of (1→4)-beta-D-glucosidic linkages in cellulose and lichenin. Cellulases are important for processing potential commercial products such as cellulosic ethanol.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

substrate

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Mari Aidemark et al.
BMC plant biology, 10, 274-274 (2010-12-16)
Alamethicin is a membrane-active peptide isolated from the beneficial root-colonising fungus Trichoderma viride. This peptide can insert into membranes to form voltage-dependent pores. We have previously shown that alamethicin efficiently permeabilises the plasma membrane, mitochondria and plastids of cultured plant
Binay Bhushan Panda et al.
BMC plant biology, 18(1), 89-89 (2018-05-23)
Poor filling of grains in the basal spikelets of large size panicles bearing numerous spikelets has been a major limitation in attempts to increase the rice production to feed the world's increasing population. Considering that biotechnological intervention could play important
Rachel A Levin et al.
The Journal of eukaryotic microbiology, 64(5), 588-597 (2017-01-26)
Dinoflagellates within the genus Symbiodinium are photosymbionts of many tropical reef invertebrates, including corals, making them central to the health of coral reefs. Symbiodinium have therefore gained significant research attention, though studies have been constrained by technical limitations. In particular
Jin Zhou et al.
Bioresource technology, 100(2), 819-825 (2008-09-06)
To improve the enzymatic hydrolytic efficiency and reduce production cost, a statistically designed experimental approach was used to optimize the composition of cellulase mixture so as to maximize the amount of glucose produced from steam-exploded corn stover (SECS). Using seven
Paula Berenstecher et al.
Current biology : CB, 30(16), 3243-3251 (2020-07-04)
Photodegradation of aboveground senescent plant material (plant litter) due to exposure to solar radiation has been identified as a dominant control on carbon (C) loss in semi-arid ecosystems [1], upturning traditional models of C cycling based only on available moisture

Protocols

To standardize an enzymatic assay procedure of cellulase.

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