C1860
Anti-Clathrin Heavy Chain antibody, Mouse monoclonal
clone TD.1, purified from hybridoma cell culture
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About This Item
Conjugate:
unconjugated
Clone:
TD.1, monoclonal
Application:
ARR, ICC, IP, WB
Citations:
10
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TD.1, monoclonal
form
buffered aqueous solution
mol wt
antigen 180-192 kDa
species reactivity
rat, human, bovine, mouse
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 2-4 μg/mL using whole extract of cultured rat adrenal pheochromocytoma PC-12 cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CLTC(1213)
mouse ... Cltc(67300)
rat ... Cltc(54241)
General description
Clathrin, a three legged structure refer as triskelion is the main component of polygonal network and forms the coat of coated vesicles and coated pits. Clathrin-coated vesicles accounts for receptor mediated endocytosis at the plasma membrane and also play a crucial role in hormone secretion and mechanism of action. Monoclonal Anti-Clathrin Heavy Chain antibody can be used to study the mechanism of clathrin mediated membrane trafficking during hormonal stimulation and protein phosphorylation. Mouse monoclonal anti-clathrin heavy chain antibodies are react specifically with clathrin heavy chain CHC17 but do not cross react with muscle isoform CHC22.
Immunogen
globular N-terminal domain of bovine clathrin heavy chain.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Clathrin Heavy Chain antibody can be used in immunoblotting. It can also be used in western blotting to characterize clathrin heavy chain levels
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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