C2542
Monoclonal Anti-N-Cadherin antibody produced in mouse
clone GC-4, ascites fluid
Synonym(s):
Anti-A-CAM, Anti-A-Cell Adhesion Molecule
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About This Item
Conjugate:
unconjugated
Clone:
GC-4, monoclonal
Application:
ARR, EM, IHC (f), WB
Citations:
16
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GC-4, monoclonal
contains
15 mM sodium azide
species reactivity
chicken, mouse, human, rabbit, rat
technique(s)
electron microscopy: suitable, immunohistochemistry (frozen sections): 1:100 using chicken cardiac muscle, microarray: suitable, western blot: 1:100 using chicken or rat cardiac muscle
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDH2(1000)
mouse ... Cdh2(12558)
rat ... Cdh2(83501)
General description
N-cadherin is a calcium dependent cell adhesion molecule and is present mainly in cancer cells. Monoclonal anti-N-cadherin antibody reacts with the N-terminal portion of the extracellular domain of adipocyte adhesion molecule (ACAM).
Immunogen
Affinity purified chicken heart A-CAM.
Application
Monoclonal anti-N-cadherin antibody can be used in immunofluorescent microscopy, electron microscopy and immunoblotting to study the receptor localization of intercellular adherens. It may also be used to study the mechanism of adhesion and interaction in tissues.
Monoclonal anti-N-cadherin antibody can be used in immunohistochemistry (1: 100) and as primary antibody in western blotting (1:1000) .
Biochem/physiol Actions
Monoclonal anti-N-cadherin antibody reacts specifically with A-CAM molecule in human, mouse, rabbit, chicken and rat.
N-cadherin regulates the Src kinase pathway and also promotes cell motility and invasion. It inhibits adherens junction formation and disrupts existing junctions of cultured lens cells.
Physical form
Monoclonal Anti-N-Cadherin antibody produced in mouse is supplied as ascites fluid containing 15mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
动物来源生物产品
常规特殊物品
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Related Content
Instructions
George Britton et al.
Methods in molecular biology (Clifton, N.J.), 2258, 119-130 (2020-12-20)
In the developing mammalian embryo, intercellular signaling allows cells to self-organize to create spatial patterns of different cell fates. This process is challenging to study because of the difficulty of observing or manipulating embryos on the spatial and temporal scales
Kamiel A J Kuijpers et al.
BioMed research international, 2014, 464217-464217 (2014-04-11)
Interstitial cells, also called myofibroblasts, most probably play a major role in the pathogenesis of the overactive bladder. However, no specific phenotypic marker has been identified. We investigated whether N-cadherin could play a role as a discriminatory marker for interstitial
Yuchang Zhou et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 247(12), 1241-1252 (2018-10-17)
Myostatin (MSTN), a member of the transforming growth factor-β (TGF-β) superfamily, has been implicated in the negative regulation of skeletal myogenesis. However, the molecular mechanism through which MSTN regulates early embryonic myogenesis is not well understood. We demonstrate that MSTN
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C2542-100UL | 04061835503827 |
| C2542-.2ML | 04061837873126 |