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Merck
CN

C2542

Monoclonal Anti-N-Cadherin antibody produced in mouse

clone GC-4, ascites fluid

Synonym(s):

Anti-A-CAM, Anti-A-Cell Adhesion Molecule

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00164511

Key Documents

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Product Name

Monoclonal Anti-N-Cadherin antibody produced in mouse, clone GC-4, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GC-4, monoclonal

contains

15 mM sodium azide

species reactivity

chicken, mouse, human, rabbit, rat

technique(s)

electron microscopy: suitable
immunohistochemistry (frozen sections): 1:100 using chicken cardiac muscle
microarray: suitable
western blot: 1:100 using chicken or rat cardiac muscle

UniProt accession no.

P19022

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... CDH2(1000)
mouse ... Cdh2(12558)
rat ... Cdh2(83501)

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Application

Monoclonal anti-N-cadherin antibody can be used in immunofluorescent microscopy, electron microscopy and immunoblotting to study the receptor localization of intercellular adherens. It may also be used to study the mechanism of adhesion and interaction in tissues.
Monoclonal anti-N-cadherin antibody can be used in immunohistochemistry (1: 100) and as primary antibody in western blotting (1:1000) .

Biochem/physiol Actions

Monoclonal anti-N-cadherin antibody reacts specifically with A-CAM molecule in human, mouse, rabbit, chicken and rat.
N-cadherin regulates the Src kinase pathway and also promotes cell motility and invasion. It inhibits adherens junction formation and disrupts existing junctions of cultured lens cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-cadherin is a calcium dependent cell adhesion molecule and is present mainly in cancer cells. Monoclonal anti-N-cadherin antibody reacts with the N-terminal portion of the extracellular domain of adipocyte adhesion molecule (ACAM).

Immunogen

Affinity purified chicken heart A-CAM.

Physical form

Monoclonal Anti-N-Cadherin antibody produced in mouse is supplied as ascites fluid containing 15mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000395438
0000388876
0000335160
0000388876
0000395438

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Exogenous expression of N-cadherin in breast cancer cells induces cell migration, invasion, and metastasis
Hazan RB
The Journal of cell biology, 148(4), 779-790 (2000)
Balázs Hegedüs et al.
Biophysical journal, 91(7), 2708-2716 (2006-07-11)
Impairment of tissue cohesion and the reorganization of the extracellular matrix are crucial events during the progression toward invasive cell phenotype. We studied the in vitro invasion patterns of nine brain tumor cell lines in three-dimensional collagen gels. Cell-cell and
Kamiel A J Kuijpers et al.
BioMed research international, 2014, 754596-754596 (2014-05-16)
The human bladder shows spontaneous autonomous activity. Detrusor overactivity could be seen as a consequence of exaggerated autonomous activity. Interstitial cells (ICs) play a potential role in coordination of autonomous activity. As it is suggested that changes in ICs coexist
Yuchang Zhou et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 247(12), 1241-1252 (2018-10-17)
Myostatin (MSTN), a member of the transforming growth factor-β (TGF-β) superfamily, has been implicated in the negative regulation of skeletal myogenesis. However, the molecular mechanism through which MSTN regulates early embryonic myogenesis is not well understood. We demonstrate that MSTN
George Britton et al.
Methods in molecular biology (Clifton, N.J.), 2258, 119-130 (2020-12-20)
In the developing mammalian embryo, intercellular signaling allows cells to self-organize to create spatial patterns of different cell fates. This process is challenging to study because of the difficulty of observing or manipulating embryos on the spatial and temporal scales
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Data Sheet - C2542

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