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Merck
CN

C2791

N-(2-Chloro-4-pyridyl)-N′-phenylurea

applicable for cell culture, BioReagent

Synonym(s):

Forchlorfenuron, 4-CPPU, CPPU

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About This Item

Empirical Formula (Hill Notation):
C12H10ClN3O
CAS Number:
68157-60-8
Molecular Weight:
247.68
NACRES:
NA.72
PubChem Substance ID:
24892531
UNSPSC Code:
10171502
MDL number:
MFCD00059898

Key Documents

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Product Name

N-(2-Chloro-4-pyridyl)-N′-phenylurea, applicable for cell culture, BioReagent

InChI

1S/C12H10ClN3O/c13-11-8-10(6-7-14-11)16-12(17)15-9-4-2-1-3-5-9/h1-8H,(H2,14,15,16,17)

SMILES string

Clc1cc(NC(=O)Nc2ccccc2)ccn1

InChI key

GPXLRLUVLMHHIK-UHFFFAOYSA-N

product line

BioReagent

form

powder

application(s)

agriculture

storage temp.

2-8°C

Quality Level

200

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Application

N-(2-Chloro-4-pyridyl)-N′-phenylurea has been used as a supplement in Murashige and Tucker medium (MT) to culture thin cell layer (TCL) explants. It has also been used to spray on to the ovaries at anthesis for induction of parthenocarpic fruit.
Forchlorfenuron (CPPU) is a cytokinin that enhances in vitro direct shoot organogenesis of Citrus aurantium L. epicotyl segments.

Biochem/physiol Actions

N-(2-Chloro-4-pyridyl)-N′-phenylurea/ CPPU, a cytokinin-like plant growth regulator participates in chlorophyll biosynthesis, cell division and cell expansion. It helps to intensify fruit set and quickens fruit enlargement. CPPU modulates carbohydrate allocation.
CPPU is a cytokinin oxidase/dehydrogenase (CKO) inhibitor.

pictograms

Health hazardEnvironment
GHS08,GHS09

signalword

Warning

hcodes

H351,H411

Hazard Classifications

Aquatic Chronic 2 - Carc. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

农药列管产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000447614
0000447614
0000427373
0000427373
0000350680

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Effect of CPPU on carbohydrate and endogenous hormone levels in young macadamia fruit
Zeng H, et al.
PLoS ONE, 11(7), e0158705-e0158705 (2016)
Celia Suárez-Pantaleón et al.
Journal of agricultural and food chemistry, 56(23), 11122-11131 (2008-11-08)
The development of immunoassays for the detection of the plant growth regulator forchlorfenuron (CPPU) is described. To achieve that purpose, a set of CPPU derivatives has been obtained by the previous synthesis of the adequate p-aminophenyl alkanoic acid. Protein conjugates
Guoying Wang et al.
Journal of plant physiology, 163(6), 591-600 (2005-12-07)
It has previously been shown that boron (B) deficiency inhibits growth of the plant apex, which consequently results in a relatively weak apical dominance, and a subsequent sprouting of lateral buds. Auxin and cytokinins (CKs) are the two most important
David Kopecný et al.
Biochimie, 92(8), 1052-1062 (2010-05-19)
Cytokinin oxidase/dehydrogenase (CKO) is a flavoenzyme, which irreversibly degrades the plant hormones cytokinins and thereby participates in their homeostasis. Several synthetic cytokinins including urea derivatives are known CKO inhibitors but structural data explaining enzyme-inhibitor interactions are lacking. Thus, an inhibitory
Antonio Valverde et al.
Journal of agricultural and food chemistry, 58(5), 2818-2823 (2010-02-10)
This paper describes the application of liquid chromatography time-of-flight mass spectrometry (LC/TOF-MS), with electrospray ionization, for residue analysis of forchlorfenuron in tomato, zucchini and watermelon. The assessed method includes a sample preparation step based on the buffered QuEChERS approach. The
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