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C2888

Sigma-Aldrich

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

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Synonym(s):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
CAS Number:
9000-81-1
Enzyme Commission number:
3.1.1.7 (BRENDA, IUBMB)
EC Number:
232-559-3
MDL number:
MFCD00081268
NACRES:
NA.32

biological source

Electrophorus electricus

Quality Level

200

type

Type V-S

form

lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

280 kDa

composition

Protein, ≥45% biuret

pI 

~5.3

solubility

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

storage temp.

−20°C

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General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
  • in acetylcholinesterase activity assay
  • to study its effects on Xenopus embryonic hippocampal cells
  • to study the effect of acetylcholine inhibitors on development and plasticity

Biochem/physiol Actions

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Caution

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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  5. Are solutions of Product C2888, Acetylcholinesterase from Electrophorus electricus (electric eel), harmed by freezing?

    Yes, solutions may be harmed by freezing. The data sheet for Product C2888 indicates that solutions stabilized by the addition of BSA are stable when kept refrigerated for up to six months. A customer has reported to us that they tried freezing a solution to which BSA was added; they found that the solution had a 40 percent loss of activity after four months in the freezer. So our best recommendation is that solutions be stabilized with BSA and kept refrigerated, not frozen.

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