C3260
Citrate Synthase from porcine heart
liquid, ≥100 units/mg protein
Synonym(s):
Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
Biological source:
Porcine heart
biological source
Porcine heart
Quality Segment
form
liquid
specific activity
≥100 units/mg protein
foreign activity
isocitrate dehydrogenase and aconitase ≤0.01%, malic dehydrogenase ≤0.1%
storage temp.
−20°C
Application
Citrate Synthase from porcine heart has been used:
- to inject newt egg for determining its importance in egg activation at fertilization
- in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
- to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs
Biochem/physiol Actions
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
Other Notes
One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.
Storage Class
12 - Non Combustible Liquids
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