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C3886

Crystal Violet

Dye content ≥90 %

Synonym(s):

Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

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About This Item

Linear Formula:
C25H30N3Cl
CAS Number:
548-62-9
Molecular Weight:
407.98
EC Number:
208-953-6
PubChem Substance ID:
57654011
UNSPSC Code:
12171500
Beilstein/REAXYS Number:
3580948
MDL number:
MFCD00011750
Colour Index Number:
42555
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form

powder

composition

Dye content, ≥90%

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

antibiotic activity spectrum

fungi

mode of action

cell membrane | interferes, enzyme | inhibits

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Application

Crystal Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. Used as a component of CV cytotoxicity assays.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBF2007V
061M18522V
SLBD6939V
061M18522
061M18521V

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Seo-Hyun Choi et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(8), 771-785 (2019-07-05)
Overexpressed Solute Carrier Family 16 Member 3 (SLC16A3, also called MCT4) plays a critical role in hypoxic cancer cell growth and proliferation, by expelling glycolysis-derived lactate across the plasma membrane. However, how SLC16A3 expression is regulated, under hypoxic conditions, is
Eleonora Grisard et al.
EBioMedicine, 46, 79-93 (2019-07-16)
Metastatic colorectal cancer (CRC) remains a deadly disease. Identifying locally advanced CRC patients with high risk of developing metastasis and improving outcome of metastatic CRC patients require discovering master regulators of metastasis. In this context, the non-coding part of the
Jae-Hoon Ji et al.
Nucleic acids research, 47(12), 6299-6314 (2019-05-03)
Histone H2AX undergoes a phosphorylation switch from pTyr142 (H2AX-pY142) to pSer139 (γH2AX) in the DNA damage response (DDR); however, the functional role of H2AX-pY142 remains elusive. Here, we report a new layer of regulation involving transcription-coupled H2AX-pY142 in the DDR.
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