C3921
Creatinase from Pseudomonas sp.
recombinant, expressed in E. coli, lyophilized powder, 10-15 units/mg protein
Synonym(s):
Creatine Amidinohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-471-1
MDL number:
Specific activity:
10-15 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
form
lyophilized powder
specific activity
10-15 units/mg protein
mol wt
45 kDa
composition
Protein, ~70% biuret
storage temp.
2-8°C
Application
Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.
Biochem/physiol Actions
Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H2O, to form urea and sarcosine.
Other Notes
One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.
Storage Class
11 - Combustible Solids
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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M-L Lee
Pediatric cardiology, 26(6), 792-796 (2005-08-06)
I report on a 3-month-old infant with pulmonary atresia-intact ventricular septum and ventriculocoronary communication (VCC) who underwent percutaneous radiofrequency valvulotomy and valvuloplasty (RFVV). The patient's cardiac troponin-I, creatine kinase (CK), and myocardial fraction of (CK-MB) were elevated before RFVV and
Jill A O'Loughlin et al.
ASAIO journal (American Society for Artificial Internal Organs : 1992), 50(3), 253-260 (2004-06-03)
An alginate microcapsule was developed that contains three enzymes (urease, uricase, and creatininase) capable of effectively degrading urea, uric acid, and creatinine, which are elevated to pathologic levels in patients with kidney failure. The capsules were evaluated in vitro and
Ashutosh Tiwari et al.
International journal of biological macromolecules, 44(5), 408-412 (2009-05-12)
A matrix made up of chitosan-SiO(2)-multiwall carbon nanotubes (CHIT-SiO(2)-MWCNTs) nanocomposite was fabricated to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-SiO(2)-MWCNTs matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-SiO(2)-MWCNTs biomatrix was characterized
Selective embolization in the management of arteriovenous fistula after renal allograft biopsy preserves renal allograft function.
Galip Guz et al.
International urology and nephrology, 37(1), 207-208 (2005-09-01)
Emine Karakuş et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 34(3), 337-347 (2006-07-01)
New potentiometric and amperometric biosensors were developed for the determination of creatine. The potentiometric creatine biosensor was prepared by immobilizing urease and creatinase on poly(vinylchloride) (PVC) ammonium membrane electrode containing palmitic acid prepared by using nonactine as an ammonium-ionophore. The
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