C-39366
Skeletal Muscle Differentiation Medium
SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml
Synonym(s):
Recommended with C-23260
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About This Item
NACRES:
NA.71
UNSPSC Code:
12352207
form
aqueous solution
packaging
pkg of 1 vial
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Skeletal Muscle Cell Differentiation Medium SupplementMix (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-23260
To form a complete medium, it should be combined with the basal medium: C-23260
Application
The Skeletal Muscle Cell Differentiation Medium is a serum-free medium developed to induce the fusion of skeletal muscle cells to myotubes with typical multinucleated syncytia. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)
Preparation Note
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Final supplement concentrations after addition to the basal medium:Insulin (recombinant human): 10 µg / ml
Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Analysis Note
All lots of PromoCell Skeletal Muscle Cell Differentiation Medium is tested for the capacity to fully induce the differentiation of SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.
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