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C4032

Sigma-Aldrich

Casein, Dephosphorylated from bovine milk

lyophilized powder

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CAS Number:
MDL number:
NACRES:
NA.61

biological source

bovine milk

Quality Level

Assay

≥85% (biuret)

form

lyophilized powder

UniProt accession no.

storage temp.

2-8°C

InChI

1S/C81H125N22O39P/c1-36(2)31-50(76(132)94-43(15-24-57(87)108)71(127)101-52(34-64(120)121)78(134)98-49(81(137)138)11-7-8-30-82)99-72(128)47(19-28-61(114)115)95-77(133)51(33-63(118)119)100-73(129)48(20-29-62(116)117)97-80(136)65(37(3)104)103-75(131)44(16-25-58(88)109)92-68(124)42(14-23-56(86)107)90-67(123)41(13-22-55(85)106)91-69(125)45(17-26-59(110)111)93-70(126)46(18-27-60(112)113)96-79(135)53(35-142-143(139,140)141)102-74(130)40(12-21-54(84)105)89-66(122)39(83)32-38-9-5-4-6-10-38/h4-6,9-10,36-37,39-53,65,104H,7-8,11-35,82-83H2,1-3H3,(H2,84,105)(H2,85,106)(H2,86,107)(H2,87,108)(H2,88,109)(H,89,122)(H,90,123)(H,91,125)(H,92,124)(H,93,126)(H,94,132)(H,95,133)(H,96,135)(H,97,136)(H,98,134)(H,99,128)(H,100,129)(H,101,127)(H,102,130)(H,103,131)(H,110,111)(H,112,113)(H,114,115)(H,116,117)(H,118,119)(H,120,121)(H,137,138)(H2,139,140,141)

InChI key

BECPQYXYKAMYBN-UHFFFAOYSA-N

Gene Information

General description

Dephosphorylated casein is prepared by the action of potato acid phosphatase. It displays improved solubility and less foaming.

Application

Casein, Dephosphorylated from bovine milk has been used as a substrate in in vitro kinase assays of Arabidopsis thaliana GSK3/Shaggy like kinase, casein kinase II and NIMA (never in mitosis A)-related kinase 2 (Nek2).

Biochem/physiol Actions

Clusters of phosphoserine in casein chelate iron and reduce its bioavailability. Partial enzymatic digestion of casein unmasks phosphorylated residues to alkaline phosphatase, permitting dephosphorylation and increasing the bioavailability of iron.
Dephosphorylated casein lacks a net negative charge and is less sensitive to calcium. It is useful in food industry.

Quality

At least 70% dephosphorylated.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The Arabidopsis thaliana GSK3/Shaggy like kinase AtSK3-2 modulates floral cell expansion
Claisse G, et al.
Plant Molecular Biology, 64(1-2) (2007)
Characterization of Cep85--a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction
Chen C, et al.
Journal of Cell Science, 128(17) (2015)
Y Mikuni-Takagaki et al.
The Biochemical journal, 268(3), 593-597 (1990-06-15)
We have detected a protein kinase which phosphorylates bone phosphoproteins (BPPs) in the detergent extract of the membranous fractions in the periosteal bone strips of 12-day-embryonic-chick tibia. This enzyme, tentatively named BPP kinase, has a catalytic subunit of Mr approximately
High-throughput chemical screen identifies a novel potent modulator of cellular circadian rhythms and reveals CKIa as a clock regulatory kinase.
Hirota T., et al.
PLoS Biology, 8 (2010)
v-Src-dependent down-regulation of the Ste20-like kinase SLK by casein kinase II
Chaar Z, et al.
Test, 281(38) (2006)

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