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C4129

α-Chymotrypsin from bovine pancreas

Name: α-Chymotrypsin from bovine pancreas
Brand: SIGMA

Type II, lyophilized powder, ≥40 units/mg protein

Synonym(s):

α-chymotrypsin A and B, alpha-chymotrypsin

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About This Item

CAS Number:

9004-07-3

UNSPSC Code:

12352204

eCl@ss:

42010112

EC Number:

232-671-2

NACRES:

NA.54

MDL number:

MFCD00130481

EC Number:

3.4.21.1 (BRENDA, IUBMB)

Specific activity:

≥40 units/mg protein

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type

Type II

Quality Level

300

form

lyophilized powder

specific activity

≥40 units/mg protein

mol wt

25 kDa

composition

protein, ≥85%

color

white to off-white

UniProt accession no.

P00767

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... CTRB1(618826)

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Related Categories

Proteases

Proteins & Enzymes

Application

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Preparation Note

Produced from 3× crystallized chymotrypsinogen

Analysis Note

Protein determined by E1%/280

Other Notes

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08,GHS07,GHS09

signalword

Danger

hcodes

H302,H315,H319,H334,H335,H400

pcodes

P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

低风险生物材料

动植物源性产品

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Certificates of Analysis (COA)

Lot/Batch Number

0000529055

0000532704

0000529056

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Partial Characterization of Protease Inhibitors of Ulva ohnoi and Their Effect on Digestive Proteases of Marine Fish.

Antonio Jesús Vizcaíno et al.

Marine drugs, 18(6) (2020-06-24)

This piece of research evaluates the presence of protease inhibitors in the macroalga Ulva ohnoi and provides an initial overview of their mode of action. The ability of Ulva protease inhibitors to inhibit digestive proteases of three marine fish species

Structure-function relationship in glycosylated alpha-chymotrypsin as probed by IMAC and IMACE.

K Y Jiang et al.

Biochimica et biophysica acta, 1433(1-2), 198-209 (1999-08-14)

Chemical glycosylation of bovine alpha-chymotrypsin, by a glucosamine adduct on the carboxyl group, results in the modification of its catalytic activity. The structural alterations of alpha-chymotrypsin resulting from its glycosylation are studied by immobilized metal-ion affinity chromatography (IMAC) and immobilized

A modified two-color flow cytometry assay to quantify in-vitro reinvasion and determine invasion phenotypes at low Plasmodium falciparum parasitemia.

Ines Atuh Ngoh et al.

Experimental parasitology, 218, 107969-107969 (2020-08-29)

Invasion of human red blood cells (RBCs) by Plasmodium parasites is a crucial yet poorly characterised phenotype. Two-color flow cytometry (2cFCM) promises to be a very sensitive and high throughput method for phenotyping parasite invasion. However, current protocols require high

A Versatile and Robust Serine Protease Inhibitor Scaffold from Actinia tenebrosa.

Xingchen Chen et al.

Marine drugs, 17(12) (2019-12-18)

Serine proteases play pivotal roles in normal physiology and a spectrum of patho-physiological processes. Accordingly, there is considerable interest in the discovery and design of potent serine protease inhibitors for therapeutic applications. This led to concerted efforts to discover versatile

Taxonomically Restricted Wheat Genes Interact With Small Secreted Fungal Proteins and Enhance Resistance to Septoria Tritici Blotch Disease.

Ciarán J Brennan et al.

Frontiers in plant science, 11, 433-433 (2020-06-02)

Understanding the nuances of host/pathogen interactions are paramount if we wish to effectively control cereal diseases. In the case of the wheat/Zymoseptoria tritici interaction that leads to Septoria tritici blotch (STB) disease, a 10,000-year-old conflict has led to considerable armaments

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Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Protocols

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

α-胰凝乳蛋白酶的酶学测定程序（EC 3.4.21.1）

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

Global Trade Item Number

SKU	GTIN
C4129-1G	04061833491683
C4129-250MG	04061833491690
C4129-10G	04061833491676
C4129-500MG	04061833491706

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