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C4765

Sigma-Aldrich

Casein solution from bovine milk

5% in water

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CAS Number:
9000-71-9
MDL number:
MFCD00081481
NACRES:
NA.61

biological source

bovine milk

Quality Level

200

form

liquid

concentration

5% in water

technique(s)

activity assay: suitable
electrophoresis: suitable
immunocytochemistry: suitable

UniProt accession no.

P02662
P02663
P02666
P02668

storage temp.

2-8°C

InChI

1S/C81H125N22O39P/c1-36(2)31-50(76(132)94-43(15-24-57(87)108)71(127)101-52(34-64(120)121)78(134)98-49(81(137)138)11-7-8-30-82)99-72(128)47(19-28-61(114)115)95-77(133)51(33-63(118)119)100-73(129)48(20-29-62(116)117)97-80(136)65(37(3)104)103-75(131)44(16-25-58(88)109)92-68(124)42(14-23-56(86)107)90-67(123)41(13-22-55(85)106)91-69(125)45(17-26-59(110)111)93-70(126)46(18-27-60(112)113)96-79(135)53(35-142-143(139,140)141)102-74(130)40(12-21-54(84)105)89-66(122)39(83)32-38-9-5-4-6-10-38/h4-6,9-10,36-37,39-53,65,104H,7-8,11-35,82-83H2,1-3H3,(H2,84,105)(H2,85,106)(H2,86,107)(H2,87,108)(H2,88,109)(H,89,122)(H,90,123)(H,91,125)(H,92,124)(H,93,126)(H,94,132)(H,95,133)(H,96,135)(H,97,136)(H,98,134)(H,99,128)(H,100,129)(H,101,127)(H,102,130)(H,103,131)(H,110,111)(H,112,113)(H,114,115)(H,116,117)(H,118,119)(H,120,121)(H,137,138)(H2,139,140,141)

InChI key

BECPQYXYKAMYBN-UHFFFAOYSA-N

Gene Information

bovine ... CSN1S1(282208) , CSN1S2(282209) , CSN2(281099) , CSN3(281728)

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General description

Casein is a calcium (phosphate) binding phosphoprotein. It is present in mammalian milk and constitutes about 80% of the total bovine milk proteins. Bovine milk consists of four casein peptides, such as αs1-,αs2-,β-, and κ-caseins. Casein exists in milk as micelles. Casein from bovine is produced by the mammary gland under multihormonal control.

Application

Casein solution from bovine milk has been used as a substrate for cyclic-AMP dependent protein kinase.
Casein solution from bovine milk has been used:
  • to determine the protease activity in ground beef and its effects on bacteriocin
  • as a non-specific substrate to measure the activity of the protein phosphatase 2C (PP2C) fusions
  • in sodium dodecyl sulfate (SDS)-polyacrylamide gel for casein zymography

Biochem/physiol Actions

Casein micelle system inhibits the pathological calcification of mammary glands. It acts as a source of amino acids for the growth of neonates.

Preparation Note

Hydrolyzed and partially dephosphorylated. Prepared from a mixture of α, β and κ caseins.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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