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C5042

Sigma-Aldrich

Cresyl Violet acetate

certified by the Biological Stain Commission

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Synonym(s):
9-Amino-5-imino-5H-benzo[a]phenoxazine acetate salt
Empirical Formula (Hill Notation):
C18H15N3O3
CAS Number:
10510-54-0
Molecular Weight:
321.33
EC Number:
234-043-3
MDL number:
MFCD00013151
PubChem Substance ID:
24892749
NACRES:
NA.47

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ~70%

color

dark green

mp

140-143 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(O)=O.Nc1cc2OC3=CC(=N)C=CC3=Nc2c4ccccc14

InChI

1S/C16H11N3O.C2H4O2/c17-9-5-6-13-14(7-9)20-15-8-12(18)10-3-1-2-4-11(10)16(15)19-13;1-2(3)4/h1-8,17H,18H2;1H3,(H,3,4)

InChI key

ORIKHDNZZCTIAD-UHFFFAOYSA-N

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General description

Cresyl violet acetate, also known as oxazine 9, is a small diaminobenzooxazine dye that has a planar conjugated system of moderate size.

Application

Cresyl Violet acetate has been used in Nissl staining of sections of the spinal cord and brain. It is also suitable for the demonstration of nuclei and Nissl substance in neuroanatomical investigations. Additional applications of Cresyl violet include staining of sex chromatin, identification of Helicobacter, and diagnostically as a vital stain in endoscopic procedures.

Suitability

Certified for Nissl staining of paraffin sections of spinal cord and brain and to stain cartilage and mast cell granules.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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