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Merck
CN

C5338

Cyanogen bromide-activated-Sepharose 4 Fast Flow

lyophilized powder

Synonym(s):

Cyanogen bromide-activated Agarose

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About This Item

CAS Number:
UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Technical Service
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form

lyophilized powder

Quality Segment

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4 Fast Flow

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 4-5 mL gel

particle size

45—165 μm

capacity

6-11 μmol/mL coupling capacity (6-AKS)

storage temp.

2-8°C

General description

C5338-10G′s update product number is GE17-0981-01
Cyanogen bromide-activated Sepharose 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.

Application

Cyanogen bromide-activated-Sepharose is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Physical form

Lyophilized powder with sugar stabilizers

Legal Information

Sepharose is a trademark of Cytiva


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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