form
lyophilized powder
Quality Segment
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4 Fast Flow
matrix active group
cyanate or related structures
matrix spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling
1 g swells to 4-5 mL gel
particle size
45—165 μm
capacity
6-11 μmol/mL coupling capacity (6-AKS)
storage temp.
2-8°C
General description
C5338-10G′s update product number is GE17-0981-01
Cyanogen bromide-activated Sepharose™ 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
Application
Cyanogen bromide-activated-Sepharose™ is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose™ has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.
Physical form
Lyophilized powder with sugar stabilizers
Legal Information
Sepharose is a trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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