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C5878

Chlorophyll b from spinach

Name: Chlorophyll b from spinach
Brand: SIGMA

≥90% (HPLC), ≤0.5% Chlorophyll a

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About This Item

Empirical Formula (Hill Notation):

C₅₅H₇₀MgN₄O₆

CAS Number:

519-62-0

Molecular Weight:

907.47

UNSPSC Code:

12352103

NACRES:

NA.47

PubChem Substance ID:

24892813

EC Number:

208-272-4

Beilstein/REAXYS Number:

4122778

MDL number:

MFCD00079053

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Quality Level

200

assay

≥90% (HPLC)

form

powder

impurities

≤0.5% Chlorophyll a

mp

183-185 °C (lit.)

ε (extinction coefficient)

15.9 × 10⁴ at 452 nm in diethyl ether (lit.), 5.61 × 10⁴ at 644 nm in diethyl ether (lit.)

application(s)

metabolomics
vitamins, nutraceuticals, and natural products

shipped in

dry ice

storage temp.

−20°C

SMILES string

[H]C(=O)c1c(CC)c2cc3c(C)c4C(=O)[C@H](C(=O)OC)c5c6nc(cc7c(C)c(C=C)c(cc1n2)n7[Mg]n3c45)[C@@H](C)[C@@H]6CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C

InChI

1S/C55H71N4O6.Mg/c1-12-38-35(8)42-27-43-36(9)40(23-24-48(61)65-26-25-34(7)22-16-21-33(6)20-15-19-32(5)18-14-17-31(3)4)52(58-43)50-51(55(63)64-11)54(62)49-37(10)44(59-53(49)50)28-46-39(13-2)41(30-60)47(57-46)29-45(38)56-42;/h12,25,27-33,36,40,51H,1,13-24,26H2,2-11H3,(H-,56,57,58,59,60,62);/q-1;+2/p-1/b34-25+;/t32-,33-,36+,40+,51-;/m1./s1

InChI key

NSMUHPMZFPKNMZ-VBYMZDBQSA-M

Related Categories

Biochemicals

Natural Products for Research and Discovery

General description

Chlorophyll b is one of the chlorophyll pigments that impart leaves their green color. Chlorophyll is a Greek name with chlorós meaning green and phyllon meaning leaf. The structure of Chlorophyll b was successfully decoded by Fischer and Wenderoth. It is present in peripheral antenna complexes along with chlorophyll a. The amount of chlorophyll b in spinach is found to be lesser than chlorophyll a.

Application

Chlorophyll b from spinach has been used:

to perform quantitative analysis of the components of carotenoid and chlorophyll in the monovarietal virgin olive oil samples
to study the function of chlorophyll and porphyrins in the photo-oxidation of dairy products using sensory evaluation and fluorescence spectroscopy
to analyze the limits of application of spectrophotometry and spectrofluorimetry in the determination of Chlorophyll a/b ratio
to examine the changes in chlorophyll -a & -b content and color of grapevine leaves

Biochem/physiol Actions

Chlorophyll b undergoes breakdown to form pheophytin b by the enzyme chlorophyllase. This causes a change in its color from green to olive-brown. It is involved in the harvesting of light energy during the process of photosynthesis and its subsequent conversion to chemical energy. Along with chlorophyll a, it plays a key role in the ability of a plant to adapt to different light intensities.

Chlorophyll b extends the absorption spectrum of light-harvesting complexes LHC-I and LHC-II above 700 nm, a region of the visible spectrum that is not absorbed when only chlorophyll a is present in the LHCs.

Packaging

Sealed ampule.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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The role of naturally occurring chlorophyll and porphyrins in light-induced oxidation of dairy products. A study based on fluorescence spectroscopy and sensory analysis

Wold JP, et al.

International dairy journal, 15(4), 343-353 (2005)

Chlorophyll and colour changes in grapevine leaves preserved by passive modification

K?rca A, et al.

European Food Research and Technology, 223(3), 387-393 (2006)

On the limits of applicability of spectrophotometric and spectrofluorimetric methods for the determination of chlorophyll a/b ratio

Kouril R, et al.

Photosynthesis Research, 62(1), 107-116 (1999)

Intraspecific variations in growth, yield and photosynthesis of sorghum varieties to ambient UV (280-400 nm) radiation.

Sunita Kataria et al.

Plant science : an international journal of experimental plant biology, 196, 85-92 (2012-09-29)

A field study was conducted to investigate the impact of ambient solar UV on the various growth, physiological and yield parameters of four sorghum (Sorghum bicolor L.) varieties-Indore-12, Indore-26, CSV-23 and Indore-27 by excluding either UV-B (<315 nm) or UV-A/B

Isolation of chlorophylls a and b from spinach by counter-current chromatography.

Carole Jubert et al.

Journal of chromatography. A, 1140(1-2), 95-100 (2006-12-14)

A method for the isolation of chlorophylls from spinach by counter-current chromatography was developed. An initial extraction protocol was devised to avoid the notorious sensitivity of chlorophylls to degradation by light, heat, oxygen, acids and bases. Further purification and separation

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Global Trade Item Number

SKU	GTIN
C5878-1MG	04061833502587
C5878-5MG	04061833502594

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