Merck
CN
All Photos(3)

Documents

C6158

Sigma-Aldrich

Crystal Violet

ACS reagent, ≥90.0% anhydrous basis

Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
Linear Formula:
C25H30N3Cl
CAS Number:
548-62-9
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
208-953-6
MDL number:
MFCD00011750
PubChem Substance ID:
57654029
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

Assay

≥90.0% anhydrous basis

form

powder

loss

≤7.5% loss on drying

mp

205 °C (dec.) (lit.)

solubility

water: soluble 50 g/L at 27 °C

density

1.19 g/cm3 at 20 °C

absorption

passes test

suitability

passes test for sensitivity as indicator

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

Mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

Signal Word

Danger

Hazard Statements

H302 - H318 - H351 - H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Plant Chromosomes (1999)
The activation of c-Jun NH2-terminal kinase is required for dihydroartemisinin-induced autophagy in pancreatic cancer cells.
Jia G, et al.
Journal of Experimental & Clinical Cancer Research, 33, 8-8 (2014)
Downregulation of survivin expression exerts antitumoral effects on mouse breast cancer cells in vitro and in vivo.
Ma WH, et al.
Oncology Letters, 11, 159-159 (2016)
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)
Carson S and Robertson D
Manipulation and Expression of Recombinant DNA (2005)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service