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C6745

Sigma-Aldrich

Collagen Type IV from human cell culture

Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

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CAS Number:
9007-34-5
EC Number:
232-697-4
MDL number:
MFCD00130825
NACRES:
NA.75

biological source

human cell culture

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

170—180 kDa

packaging

pkg of 1 mL

concentration

0.3 mg/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

impurities

HBSAG, none detected
 HCV, none detected
 HIV-1/HIV-2, none detected

NCBI accession no.

NM_001845.6

UniProt accession no.

P02462

Binding Specificity

Peptide Source: Laminin

Peptide Source: Nidogen

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COL4A1(1282)

Related Categories

General description

COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats . It is an important constituent of type IV collagen in the basement membrane . The COL4A1 gene is located on human chromosome 13q34 .
Collagen IV, derived from human fibroblasts and epithelial cells in a co-culture system, creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for three dimensional cell culture.

Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.

Application

Collagen Type IV from human cell culture has been used in iPSC (induced pluripotent stem cells) culture and differentiation. It has also been used as membrane coatings.

Biochem/physiol Actions

COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.

Features and Benefits

  • This Collagen Type IV product is generated by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro.
  • The Collagen Type IV is then purified biochemically.
  • It is sterilized by dialysis in an acetic acid and 0.5% chloroform solution and is supplied sterile at a concentration of ~0.3 mg/ml in 25% acetic acid.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Epidemiologically and clinically relevant Group B Streptococcus isolates do not bind collagen but display enhanced binding to human fibrinogen.
Dramsi S, et al.
Microbes and Infection, 14(12), 1044-1048 (2012)
Novel mutations in three families confirm a major role of COL4A1 in hereditary porencephaly.
Breedveld G, et al.
Journal of medical Genetics, 43(6), 490-495 (2006)
Telomere length defines the cardiomyocyte differentiation potency of mouse induced pluripotent stem cells.
Aguado T, et al.
Stem Cells, 35(2), 362-373 (2017)
Chiyi Xiong et al.
Scientific reports, 10(1), 520-520 (2020-01-18)
The tyrosine kinase receptor EphB4 is frequently overexpressed in ovarian and other solid tumors and is involved in interactions between tumor cells and the tumor microenvironment, contributing to metastasis. Trans-interaction between EphB4 and its membrane-bound ligand ephrin B2 (EFNB2) mediates
Cerebrovascular disease related to COL4A1 mutations in HANAC syndrome.
Alamowitch S, et al.
Neurology, 73(22), 1873-1882 (2009)
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