C6788
4-Chloro-1-naphthol
peroxidase substrate, tablet, suitable for use in immunoblotting procedures
Synonym(s):
4-CN, 4-chloronaphthalen-1-ol
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About This Item
Linear Formula:
ClC10H6OH
CAS Number:
Molecular Weight:
178.61
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
210-068-5
MDL number:
Beilstein/REAXYS Number:
509750
Product Name
4-Chloro-1-naphthol, tablet
form
tablet
Quality Segment
mp
118-121 °C (lit.)
solubility
methanol: 10 mL, clear, colorless
storage temp.
−20°C
SMILES string
Oc1ccc(Cl)c2ccccc12
InChI
1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
InChI key
LVSPDZAGCBEQAV-UHFFFAOYSA-N
Application
4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.
Biochem/physiol Actions
4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.
Preparation Note
Contains 30 mg substrate per tablet.
Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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