C7464
Monoclonal Anti-Cyclin D1 antibody produced in mouse
clone DCS-6, ascites fluid
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About This Item
Conjugate:
unconjugated
Clone:
DCS-6, monoclonal
Application:
ARR, ICC, IHC (f), IHC (p), IP, WB
Citations:
25
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
DCS-6, monoclonal
mol wt
antigen 36 kDa (several lower and higher molecular weight bands)
contains
15 mM sodium azide
species reactivity
rat, mouse, human, monkey
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 1:200 using a human tumor cell line extract
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)
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General description
Monoclonal Anti-Cyclin D1 (mouse IgG2a isotype) is derived from the DCS-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin D1 protein is a G1-phase cyclin in mammalian cells. This protein is a D-type cyclin. The CCD1 gene is located on the human chromosome at 11q13.3.
Immunogen
recombinant human Cyclin D1 protein.
Application
Monoclonal Anti-Cyclin D1 antibody produced in mouse may be used in:
- immunoblotting
- immunofluorescence
- immunocytochemistry
- immunoprecipitation
- immunohistochemistry
- antibody-mediated knock-out experiments
Biochem/physiol Actions
Cyclin D1 is involved in regulating cyclin-dependent kinase (CDK) family members. Cyclin D1 and D2 are affiliated with CDK4 and/or CDK6 kinase activities that are observed in mid-G1 and culminate in the late G1 phase. Amplification of the cyclin D1 gene is observed in a significant percentage of cancers, including breast, bladder carcinoma, mantle cell lymphomas, head and neck cancers. This gene is also amplified in esophageal squamous cell carcinomas.
Does not cross-react with other D-type cyclins.
Physical form
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Mu Yao et al.
The British journal of nutrition, 108(3), 424-430 (2011-11-10)
We have developed a blend of food extracts commonly consumed in the Mediterranean and East Asia, named blueberry punch (BBP), with the ultimate aim to formulate a chemoprevention strategy to inhibit prostate cancer progression in men on active surveillance protocol.
Takeshi Shimamura et al.
Cancer research, 65(14), 6401-6408 (2005-07-19)
Somatic mutations in the kinase domain of the epidermal growth factor receptor (EGFR), including L858R and exon 19 deletions, underlie responsiveness to gefitinib and erlotinib in non-small cell lung cancer (NSCLC). Acquired resistance to these tyrosine kinase inhibitors is in
H Nakagawa et al.
Cancer, 76(4), 541-549 (1995-08-15)
Oncogene activation and tumor suppressor gene inactivation have been implicated in the genetic basis of esophageal squamous cell carcinoma (ESCC). Cyclin D1, an oncogene that has a critical role in G1 progression of the cell cycle, has been observed to
Huei-Tzu Chien et al.
Cancers, 11(6) (2019-06-05)
Oral squamous cell carcinoma (OSCC) is a common cancer in Taiwan and worldwide. To provide some clues for clinical management of OSCC, 72 advanced-stage OSCCs were analyzed using two microarray platforms (26 cases with Affymetrix 500 K and 46 cases
Yanhong Yang et al.
Oncology letters, 15(4), 5280-5284 (2018-03-20)
Nimotuzumab, a humanized IgG1 monoclonal antibody against epidermal growth factor receptor (EGFR), increases radiosensitivity in lung cancer. Cisplatin is an effective antitumor agent in lung cancer. In the present study, the antitumor activity of nimotuzumab combined with cisplatin was investigated
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Datasheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C7464-.5ML | 04061837867859 |
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