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Sigma-Aldrich

Creatine Phosphokinase from bovine heart

Type III, salt-free, lyophilized powder, ≥30 units/mg protein

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Synonym(s):
ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
CAS Number:
9001-15-4
Enzyme Commission number:
2.7.3.2 (BRENDA, IUBMB)
EC Number:
232-585-5
MDL number:
MFCD00130876
eCl@ss:
32160410
NACRES:
NA.54

biological source

bovine heart

Quality Level

200

description

Contains primarily isozyme MM

type

Type III

form

salt-free, lyophilized powder

specific activity

≥30 units/mg protein

mol wt

80 kDa

storage temp.

−20°C

Related Categories

Application

Creatine Phosphokinase from bovine heart has been used:
  • in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria
  • in autoubiquitination assay
  • in the preparation of premix buffer for adenylyl cyclase activity
  • in the preparation of E-mix for in vitro nuclear assembly and isolation

Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves.
The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.

Biochem/physiol Actions

Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.

Caution

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

Unit Definition

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

Analysis Note

Protein determined by biuret.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for western blotting and scanning electron microscopy (SEM)
Allen TD, et al.
Nature Protocols, 2(5), 1173-1173 (2007)
P K Clausen et al.
Journal of forensic sciences, 25(4), 765-778 (1980-10-01)
A pyrolysis-gas-liquid chromatographic method has been developed for the differentiation of adult and fetal bloodstains. In a blind-coded study, five adult and three fetal bloodstains were correctly identified on the basis of the pyrograms of stain extracts. The differentiation between
Isolation, purification and characterization of creatine kinase from bovine cardiac muscle.
O S Herasymowych et al.
Biochimica et biophysica acta, 534(1), 38-47 (1978-05-24)
M S Fehrs et al.
Journal of dairy science, 64(8), 1700-1706 (1981-08-01)
Sixteen male Holstein calves in a 2 X 2 factorial design with four animals per treatment were fed 0 and 100 ppm supplemental copper from copper carbonate and 0 and 1 ppm added selenium from sodium selenite for 15 days
A C Galloway et al.
Surgery, 96(2), 435-439 (1984-08-01)
The development of myocardial ischemia is known to elicit the formation and enlargement of collateral vessels. The stimulus for these events is unknown. We have investigated the possibility that cardiac tissue releases a factor that can stimulate endothelial cell proliferation.
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