form
beads
Quality Segment
matrix
1% cross-linked polystyrene, cross-linked polystyrene beads
matrix active group
iminodiacetic acid (sodium form), paired iminodiacetate groups
matrix attachment
through methyl group to aromatic rings
particle size
50-100 mesh (dry)
pH
4-14
capacity
≥0.3 meq/mL binding capacity, 0.6 meq/g binding capacity (heavy metal ions)
Application
Chelex® 100 sodium form已用于:
- 从蜜蜂样本中提取DNA
- 去除香烟烟雾提取物(CSE)并研究铁调素在小鼠肺组织中表达的影响
- 从细菌培养物中提取DNA
Chelex已用于亲和层析、蛋白质层析、螯合树脂、亲和树脂以及核心生物试剂。
Biochem/physiol Actions
Chelex® 100树脂是含有配对亚氨基二乙酸离子(在结合多价金属离子过程中用作螯合基团)的苯乙烯-二乙烯基苯共聚物。它归为弱阳离子型树脂。Chelex作为一种螯合树脂,用于从法医样本(如头发、血迹卡和口腔拭子)中提取DNA。
Other Notes
钠形式产品可进行高压蒸汽灭菌。最高工作温度75°C ,不溶于水。
Legal Information
Chelex is a registered trademark of Bio-Rad Laboratories, Inc.
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)